Fc gamma RIIA/CD32a Antibody (7.3), Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP2447210.2MG

Catalog No. NBP24472102

Description

Ensure accurate, reproducible results in Flow Cytometry, Immunofluorescence

Fc gamma RIIA/CD32a Monoclonal antibody specifically detects Fc gamma RIIA/CD32a in Human samples. It is validated for Flow Cytometry, SDS-Page, Immunofluorescence.

Specifications

• Antigen: Fc gamma RIIA/CD32a
• Classification: Monoclonal
• Concentration: 0.2 mg/mL
• Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, SDS-Page, Immunofluorescence 0.5 - 1.0 ug/ml
• Gene Accession No.: P12318
• Gene Symbols: FCGR2A
• Immunogen: K562 and FcgRII+L cells
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Gene ID (Entrez): 2212
• Target Species: Human
• Form: Purified

• Applications: Flow Cytometry, SDS-Page, Immunofluorescence
• Clone: 7.3
• Conjugate: Unconjugated
• Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene Alias: CD32 antigen, CD32A, CD32MGC23887, CDw32fc-gamma-RIIa, Fc fragment of IgG, low affinity IIa, receptor (CD32), Fc fragment of IgG, low affinity IIa, receptor for (CD32), FCG2, Fc-gamma RII-a, Fc-gamma-RIIa, FcGR, FCGR2, FCGR2A1, FcRII-a, IGFR2MGC30032, IgG Fc receptor II-a, Immunoglobulin G Fc receptor II, low affinity immunoglobulin gamma Fc region receptor II-a
• Host Species: Mouse
• Molecular Weight of Antigen: 40 kDa
• Quantity: 0.2 mg
• Primary or Secondary: Primary
• Test Specificity: This MAb reacts with a CD32 (FcgRII) epitope (cluster-4). It displays a stronger reaction with Daudi than with U937 cells. The epitope is located in domain 2 of FcgRIIa. Its Fab'2 fragments block immune complex binding. CD32 (Fc?RII) is a type 1 transmembrane glycoprotein that mediates several functions including phagocytosis, cytotoxicity, and immunomodulation as well as platelet aggregation. Three genes (A, B, and C) encode CD32 and at least 6 isoforms are generated via alternative mRNA splicing, i.e., IIa1, IIa2, IIb1, IIb2, IIb3 and IIc. Monocytes/macrophages, placental trophoblasts and endothelial cells express all isoforms. In addition, the IIb isoform is expressed by B cells, and the IIa isoform by platelets, granulocytes and, weakly, by B cells. NK cells and neutrophils express Isoform IIc. CD32 binds weakly to the Fc region of monomeric IgG but more strongly to IgG aggregates and immune complexes.
• Content And Storage: Store at 4C.
• Isotype: IgG1 κ

For Research Use Only