Thermo Scientific™ HpyF3I (DdeI) 

The HpyF3I (DdeI) restriction enzyme recognizes C^TNAG sites and cuts best at 37°C in Tango buffer (Isoschizomers: BstDEI, DdeI).

Manufacturer:  Thermo Scientific™ ER1882

Catalog No. FERER1882

Description

Lambda DNA digested with HpyF3I (DdeI), 1.4% agarose, 104 cleavage sites

conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

5'..C▵T N A G...3'

3'..G A N T▵C...5'

Conditions for 100% Activity:

• 1X Buffer Tango™: 33mM Tris-acetate (pH 7.9 at 37°C), 10mM Mg-acetate, 66mM K-acetate and 0.1mg/mL BSA
• Incubate at 37°C

Storage Buffer:

• HpyF3I is supplied in: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM DTT, 1mM EDTA, 0.2mg/mL BSA and 50% (v/v) glycerol

Methylation Effects:

• Dam: never overlaps —no effect
• Dcm: never overlaps —no effect
• CpG: never overlaps —no effect
• EcoKI: never overlaps —no effect
• EcoBI: may overlap —effect not determined

Compatible Ends:

• BbvCI, Bpu10I, Bpu1102I, Eco81I

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Specifications

• Product Type: HpyF3I (DdeI)
• Concentration: 10U/μL
• Quantity: 2,500U