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Thermo Scientific™ RevertAid First Strand cDNA Synthesis Kit
GSA_VA

Perform efficient synthesis of first strand cDNA from RNA templates with this complete synthesis system.

$122.00 - $533.00

Specifications

Content And Storage The RevertAid First Strand cDNA Synthesis Kit contains RevertAid Reverse Transcriptase, RiboLock RNase Inhibitor, 5X Reaction Buffer, dNTP Mix, Oligo(dT)18 Primer, Random Hexamer Primer, Control GAPDH RNA, 10 μM Forward GAPDH Primer, 10 μM Reverse GAPDH Primer, and nuclease-free water.
Store at -20°C.
Final Product Type cDNA (First-Strand)
PCR Method RT-PCR
Reaction Format Kit
Reagent Type Reverse Transcription
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Products 2
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FERK1622
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Description

Description

RevertAid First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates. The kit is suitable for synthesis of cDNA up to 13 kb. RiboLock RNase Inhibitor, supplied with the kit, effectively protects RNA templates from degradation.

The RevertAid First Strand cDNA Synthesis Kit is supplied with both oligo(dT)18 and random hexamer primers. The oligo(dT)18 primer anneals selectively to the poly(A) tail of mRNA. Random hexamer primers do not require the presence of the poly(A) tail, therefore, they can be used for transcription of the 5'-end regions of mRNA or cDNA synthesis of RNA species lacking a poly(A) tail (e.g., microRNAs). Gene-specific primers may also be used with the kit.

Highlights

  • Full-length first strand cDNA up to 13 kb
  • Optimum reaction temperature 42°C
  • Complete kit -all the components for the RT reaction are included

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Specifications

Specifications

The RevertAid First Strand cDNA Synthesis Kit contains RevertAid Reverse Transcriptase, RiboLock RNase Inhibitor, 5X Reaction Buffer, dNTP Mix, Oligo(dT)18 Primer, Random Hexamer Primer, Control GAPDH RNA, 10 μM Forward GAPDH Primer, 10 μM Reverse GAPDH Primer, and nuclease-free water.
Store at -20°C.
RT-PCR
Reverse Transcription
Up to 13 kb
Reverse Transcription
RevertAid
RevertAid
cDNA (First-Strand)
Kit
RNA
RNA
42°C
First Strand CDNA Synthesis Kit
Real-Time Quantitative PCR (qPCR), Reverse Transcriptase PCR (RT-PCR)
SDS
Product Certifications
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