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Thermo Scientific™ RevertAid H Minus Reverse Transcriptase (200 U/µL) Available on GSA/VA Contract for Federal Government customers only.

A recombinant M-MuLV RT lacking RNase H activity for high yield cDNA synthesis.

$122.20 - $497.00


Products 2
Catalog Number Mfr. No. Includes Price Quantity & Availability  
Catalog Number Mfr. No. Includes Price Quantity & Availability  
FEREP0451
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Thermo Scientific™
EP0451
1mL 5X Reaction Buffer Each for $122.20
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FEREP0452 Available on GSA/VA Contract for Federal Government customers only.
View Documents
Thermo Scientific™
EP0452
5 x 1mL 5X Reaction Buffer Each for $497.00
Add to cart
 
Description

Description

RevertAid H Minus Reverse Transcriptase is a recombinant M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks RNase H activity due to a point mutation in the RNase H domain. RevertAid H Minus Reverse Transcriptase does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA, and therefore high yields of full-length cDNA from long templates are obtained. RevertAid H Minus Reverse Transcriptase maintains activity over a wide temperature range (42 to 50°C).

Highlights

  • High yields of full-length first strand cDNA up to 13 kb
  • Optimum activity at 42 to 45°C
  • Active up to 55°C
  • Incorporates modified nucleotides (e.g., Cy3-, Cy5-, rhodamine-, aminoallyl-, fluorescein-labeled nucleotides)

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