Promotional price valid on web orders only. Your contract pricing may differ. Interested in signing up for a dedicated account number?
Learn More

Thermo Scientific™ RevertAid H Minus Reverse Transcriptase (200 U/μL)

Thermo Scientific RevertAid H Minus Reverse Transcriptase is a recombinant M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks RNase H activity due to a point mutation in the RNase H domain.

$171.00 - $546.65

Specifications

Content And Storage

• RevertAid H Minus Reverse Transcriptase (200 U/μL)
• 5X Reaction Buffer

Store at –20°C.

Format Stand-alone Enzyme
Reaction Speed 60 min.
Technique Reverse Transcription
Optimal Reaction Temperature 42°C to 45°C
View More Specs

Products 2
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
FEREP0451
View Documents
Thermo Scientific™
EP0451
10,000 Units
Each for $171.00
Add to Cart
 
FEREP0452
View Documents
Thermo Scientific™
EP0452
5 x 10,000 Units
Each for $546.65

Save 21%

Reg : $691.00

The following deviations and disclosures may produce prices below stated discounts but will not trigger price reductions: Price shown reflects a temporary, promotional price reduction. No other purchase or commercial obligation required to receive the reduced price. Price may change at any time. Promotional price valid on web orders only. Your contract pricing may differ. Other exclusions apply.
Learn More
Add to Cart
 
Description

Description

Thermo Scientific RevertAid H Minus Reverse Transcriptase is a recombinant M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks RNase H activity due to a point mutation in the RNase H domain. RevertAid H Minus Reverse Transcriptase does not degrade RNA in RNA-DNA hybrids during synthesis of the first strand cDNA, and therefore high yields of full-length cDNA from long templates are obtained. RevertAid H Minus Reverse Transcriptase maintains activity over a wide temperature range (42–50°C).

Features of RevertAid H Minus Reverse Transcriptase include:
• High yields of full-length first strand cDNA up to 13 kb
• Optimum activity at 42–45°C
• Active up to 55°C
• Incorporates modified nucleotides (e.g., Cy3-, Cy5-, rhodamine-, aminoallyl-, and fluorescein-labeled nucleotides)

Applications
• First strand cDNA synthesis for RT-PCR and real-time RT-PCR
• Reverse transcription at elevated temperatures to reduce effects of secondary structure
• Synthesis of cDNA for cloning and expression
• Generation of labeled cDNA probes for microarrays
• DNA labeling
• Analysis of RNA by primer extension

Specifications

Specifications

• RevertAid H Minus Reverse Transcriptase (200 U/μL)
• 5X Reaction Buffer

Store at –20°C.

60 min.
42°C to 45°C
Reduced
200 U/μL
Separate components
Up to 13 kb
Stand-alone Enzyme
Reverse Transcription
RevertAid H Minus
Dry Ice
First-Strand cDNA
Reverse Transcription
RNA
SDS
Documents

Documents

Product Certifications

For Research Use Only. Not for use in diagnostic procedures.

Provide Content Correction

We continue to work to improve your shopping experience and your feedback regarding this content is very important to us. Please use the form below to provide feedback related to the content on this product.

Product Title

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.

Cancel Submit