FGFR-4, Rabbit anti-Human, Clone: 29.2, Millipore Sigma™

Rabbit Monoclonal Antibody

Supplier:  MilliporeSigma MABC112725UL

Catalog No. MABC112725

Description

Anti-FGFR-4, clone 29.2, Cat. No. MABC1127, is a rabbit monoclonal antibody that detects Fibroblast growth factor receptor 4 and has been tested for use in Flow Cytometry.

Fibroblast growth factor receptor 4 (UniProt: P22455; also known as EC: 2.7.10.1, FGFR-4, CD334) is encoded by the FGRF4 (also known as JTK2, TKF) gene (Gene ID: 2264) in human. FGFR-4 is a highly conserved, single-pass type I membrane protein with tyrosine kinase activity that serves as a cell surface receptor for fibroblast growth factors and regulates cell proliferation differentiation, and migration. FGFR-4 is expressed in gastrointestinal epithelial cells, pancreas, and gastric and pancreatic cancer cell lines. In the absence of a ligand, it is present as a monomer in an inactive conformation. Upon ligand binding it homodimerizes into an active conformation with autophosphorylation of tyrosine residues. FGFR-4 is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off in the mature form. It contains an extracellular domain (aa 22-369), a transmembrane domain (aa 370-390), and a cytoplasmic domain (aa 391-802). FGFR-4 undergoes N-glycosylation, and when not fully glycosylated it can undergo proteasomal degradation. FGFR-4 signaling is down-regulated by receptor internalization and degradation. MMP14 is reported to promote internalization and degradation of FGFR-4. Mutations that lead to constitutive kinase activation or impair normal FGFR-4 inactivation lead to aberrant signaling leading to tumor growth. (Ref.: Turkington, RC et al. (2014). Cell Death Dis. 5; e1046).

Specifications

• Antigen: FGFR-4
• Classification: Monoclonal
• Conjugate: Unconjugated
• Formulation: Rabbit monoclonal antibody in supernatant without preservatives.
• Gene Symbols: FGFR4
• Immunogen: His-tagged recombinant extracellular domain of human fibroblast growth factor receptor 4 (FGFR-4).
• Quantity: 25 μL
• Research Discipline: Signaling
• Gene ID (Entrez): NP_001278909
• Target Species: Human
• Form: Supernatant

• Applications: Flow Cytometry
• Clone: 29.2
• Dilution: Flow Cytometry Analysis: A 1:4 dilution from a representative lot detected FGFR-4 in one million LNCaP cells.
• Gene Alias: Fibroblast growth factor receptor 4;EC: 2.7.10.1;CD334
• Host Species: Rabbit
• Purification Method: Unpurified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: This rabbit monoclonal antibody detects human fibroblast growth factor receptor 4 (FGFR-4). It targets an epitope with in the extracellular domain.
• Content And Storage: Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
• Isotype: IgG