Fibronectin Antibody (DH1), Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP151723

Catalog No. NBP151723

Description

Fibronectin Monoclonal specifically detects Fibronectin in Human, Rat, Chicken, Guinea Pig, Rabbit samples. It is validated for Western Blot, Immunohistochemistry, Immunohistochemistry-Paraffin.

Specifications

• Antigen: Fibronectin
• Classification: Monoclonal
• Concentration: 0.1 mg/mL
• Dilution: Western Blot 1:100-1:2000, Immunohistochemistry 1:10-1:500, Immunohistochemistry-Paraffin 1:10-1:500
• Gene Symbols: FN1
• Immunogen: The antibody reacts with Fibronectin.
• Quantity: 0.025 mg
• Research Discipline: Cellular Markers, Extracellular Matrix, Mesenchymal Stem Cell Markers, Neuronal Stem Cell Markers, Stem Cell Markers
• Gene ID (Entrez): 2335
• Target Species: Human, Rat, Chicken, Guinea Pig, Rabbit
• Form: Purified

• Applications: Western Blot, Immunohistochemistry, Immunohistochemistry (Paraffin)
• Clone: DH1
• Conjugate: Unconjugated
• Gene Alias: CIG, ED-B, fibronectin 1, FINC, FN, FN1, FNZ, GFND, GFND2, LETS, MSF, SMDCF
• Host Species: Mouse
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: The antibody is specific to extradomain A (EDA) sequence of a cellular fibronectin and recognizes thus only the cellular fibronectin. Mab DHI has been shown to specifically block chondrocyte condensation in chicken embryos (5). The antibody cross reacts with human, chicken, rat, rabbit and guinea pig. Fibronectins are diverse high molecular weight cell adhesion glycoproteins found in basement membranes, in the interstitial connective tissue matrix, and in soluble in plasma and other body fluids. In adult tissues most fibronectin is produced by hepatocytes (pFn) that differ from locally produced cellular fibronectins. A monoclonal antibody (BF12) has been produced to fibronectin that reacts with both the cellular and plasma forms of the protein and recognizes the cell-binding region in fibronectin. The monoclonal antibody DH1 recognizes only cellular form of Fibronectin. DH1 is specific to the extra domain A (EDA) of the molecule. cFns are found in some basement membranes during development and only in distinct tissues in adult, for instance in smooth muscle and alveolar walls of airways. cFns are abundant in the plasma of carcinoma patients and in the stroma of various carcinomas. Monoclonal antibody (Mab) to cFn is derived from the hybridoma produced by fusion between myeloma cells and Balb/c spleen cells. Purified fibronectin from A8387 fibrosarcoma cells was used as an immunogen (21).
• Content And Storage: Store at 4C. Do not freeze.
• Isotype: IgG1

For Research Use Only