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Cytiva Ficoll PM400 Available on GSA/VA Contract for Federal Government customers only.

High molecular weight sucrose-polymers formed by copolymerization of sucrose with epichlorohydrin. Cytiva™ Ficoll PM400 is a synthetic neutral, highly-branched hydrophilic polymer of sucrose, used for producing density gradients for separation of cells and subcellular components by centrifugation or by sedimentation at unit gravity.

$300.00 - $7450.00

Specifications

Product Type Centrifugation Media
For Use With (Application) Cell isolation
Format Powder
Density 1.2 g/mL
Storage Requirements 4–30°C
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Products 3
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
Catalog Number Mfr. No. Quantity Price Quantity & Availability  
45-001-745
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Cytiva
17030010
100 g
Each for $300.00
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45-001-746
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Cytiva
17030050
500 g
Each for $1,221.00
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45-001-792 Available on GSA/VA Contract for Federal Government customers only.
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Cytiva
17030005
5 kg
Each for $7,450.00
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Description

Description

  • For producing density gradients for separation of cells and subcellular components by centrifugation or by sedimentation at unit gravity
  • Neutral, highly branched, hydrophilic polymer of sucrose which dissolves readily in aqueous solution
  • Concentrations of up to 50% (w/v) covering densities of up to 1.2g/mL can be achieved
  • Has better osmotic properties than sucrose
  • Preserves functional and morphological integrity
  • Useful for separating cells that are sensitive to centrifugation and for separating cells of similar density but different size
  • Does not penetrate biological membranes
  • Serves as the raw material for preparation of Ficoll-Paque gradients
  • Used in other applications such as electrophoresis, hybridization, cryopreservation, and as a hapten carrier
  • Supplied as dry powders
  • Used in the formation of density gradients for the separation and isolation of eukaryotic cells, organelles, and bacterial cells, as a stabilizing agent and as a separation medium for the isolation of lymphocytes
  • Used in defined culture media, nucleic acid hybridization, electrophoresis, and immunological studies
  • Used for gradient centrifugation in all types of centrifuge rotors and for separation at unit gravity

Specifications

Specifications

Centrifugation Media
Powder
4–30°C
5 ηr at 20°C: 10% (w/v), 20 ηr at 20°C: 20% (w/v), 60 ηr at 20°C : 30% (w/v, 180 ηr at 20°C : 40% (w/v) , 600 ηr at 20°C : 50% (w/v)
Cell isolation
1.2 g/mL
0.12 EU/mg
Safety and Handling

Safety and Handling

ShelfLife : 5 years

SDS
Product Certifications
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