Invitrogen™ eBioscience™ Fixable Viability Dye eFluor™ 660

Description

Can be used to irreversibly label dead cells prior to cryopreservation, fixation and/or permeabilization procedures. Unlike 7-AAD and PI, cells labeled with Fixable Viability Dyes can be washed, fixed, permabilized and stained for intracellular antigens without loss of staining intensity.

• Fixable Viability Dyes may be used to label cells from all species
• Fixable Viability Dye eFluor™ 660 can be excited by the red (633nm) laser line and has a peak emission of 660nm that can be detected using a 660/20 band pass filter (equivalent to APC or Alexa Fluor™ 647)
• For compensation, it is recommended to use a sample of the cells of interest stained with the Fixable Viability Dye
• If the percentage of dead cells is expected to be less than 5%, then it is recommended to take a small aliquot of cells and heat them at 65°C for 1 minute then immediately place on ice for 1 minute. After this treatment, the heat-killed cells can be combined 1:1 with live cells and then stained with the Fixable Viability Dye.
• Fixable Viability Dye eFluor™ 660 is supplied as a pre-diluted solution prepared in high-quality, anhydrous DMSO. It should be protected from light and moisture
• Store at −70°C with dessicant
• It may be freeze-thawed up to 20 times
• Allow vial to equilibrate to room temperature before opening
• Staining may be done before or after surface staining
• Cells may be cryopreserved after staining with no adverse effect on staining intensity of dead cells after thawing

Specifications

• Color: Red
• Detection Method: Fluorescence
• For Use With (Application): Viability Assay
• For Use With (Equipment): Flow Cytometer
• Product Type: Dye
• Emission: 633/660
• Product Line: eBioscience
• Quantity: 500 tests

For Research Use Only. Not for use in diagnostic procedures.