Invitrogen eBioscience Fixable Viability Dye eFluor 660
Can be used to irreversibly label dead cells prior to cryopreservation, fixation and/or permeabilization procedures. Unlike 7-AAD and PI, cells labeled with Fixable Viability Dyes can be washed, fixed, permabilized and stained for intracellular antigens without loss of staining intensity.
Manufacturer: Invitrogen 65086418
- Fixable Viability Dyes may be used to label cells from all species
- Fixable Viability Dye eFluor 660 can be excited by the red (633nm) laser line and has a peak emission of 660nm that can be detected using a 660/20 band pass filter (equivalent to APC or Alexa Fluor 647)
- For compensation, it is recommended to use a sample of the cells of interest stained with the Fixable Viability Dye
- If the percentage of dead cells is expected to be less than 5%, then it is recommended to take a small aliquot of cells and heat them at 65°C for 1 minute then immediately place on ice for 1 minute. After this treatment, the heat-killed cells can be combined 1:1 with live cells and then stained with the Fixable Viability Dye.
- Fixable Viability Dye eFluor 660 is supplied as a pre-diluted solution prepared in high-quality, anhydrous DMSO. It should be protected from light and moisture
- Store at -70°C with dessicant
- It may be freeze-thawed up to 20 times
- Allow vial to equilibrate to room temperature before opening
- Staining may be done before or after surface staining
- Cells may be cryopreserved after staining with no adverse effect on staining intensity of dead cells after thawing
|Flow Cytometric Analysis|
|DMSO, pre-diluted to test size|
|≤−70°C. Protect from light and moisture.|
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