GABA A Receptor alpha 6, Rabbit anti-Rat, Bovine, Polyclonal, Millipore Sigma™

Rabbit Polyclonal Antibody

Catalog Number	Mfr. No.	Quantity
AB5610I25UG	MilliporeSigma AB5610I25UG	25 μg
AB5610I100	MilliporeSigma AB5610I100UG	100 μg

Description

Anti-GABA A Receptor alpha 6, Cat. No. AB5610-I, is a highly specific rabbit polyclonal antibody that targets Gamma-aminobutyric acid type A receptor alpha6 subunit and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Gamma-aminobutyric acid type A receptor alpha 6 subunit (UniProt: E1BE96; also known as GABA A receptor alpha 6) is encoded by the GABRA6 gene (Gene ID: 539194) in bovine. GABA is the major inhibitory neurotransmitter in the central nervous system that inhibits the generation of the action potential of the neuron. It is involved in the pathogenesis of certain neurological and psychiatric disorders. GABA is produced from glutamic acid in a reaction catalyzed by glutamic acid decarboxylase. GABA interacts with the GABAA and GABAB receptors, which are widely distributed throughout the nervous system and in a variety of cell types. They differ in their pharmacological, electrophysiological, and biochemical properties. GABAA-receptor are pentameric membrane proteins that mediate an increase in membrane conductance with an equilibrium potential near the resting level of 70 mV. This conductance increase often is accompanied by a membrane hyperpolarization, which later results in a reduction in the probability of action potential initiation. The reduction in membrane resistance is accomplished by the GABA-dependent facilitation of chloride ion influx. Mos GABA A receptors contain one type of alpha and beta subunit, and a single gamma polypeptide in a ratio of 2:2:1. GABA A receptor alpha 6 subunit is synthesized with a signal peptide (aa 1-19), which is cleaved off in the mature form. It contains four transmembrane domains (aa 240-262, 274-293, 305-328, and 423-441).

Specifications

• Antigen: GABA A Receptor alpha 6
• Applications: Immunohistochemistry (Paraffin), Western Blot
• Conjugate: Unconjugated
• Regulatory Status: RUO
• Target Species: Rat, Bovine
• Gene Alias: Gamma-aminobutyric acid type A receptor alpha6 subunit
• Gene Symbols: GABRA6
• Primary or Secondary: Primary
• Content And Storage: Stable for 1 year at 2-8°C from date of receipt.
• Molecular Weight of Antigen: ∽51 kDa observed; 51.03 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

• Dilution: Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution from a representative lot detected GABA A Receptor alpha 6 in mouse cerebellum tissue sections.
• Classification: Polyclonal
• Host Species: Rabbit
• Research Discipline: Neuroscience
• Formulation: Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
• Gene ID (Entrez): NM_000811.2
• Immunogen: KLH-conjugated linear peptide corresponding to 16 amino acids from the N-terminal region of bovine GABA A receptor alpha 6 subunit.
• Purification Method: Affinity Purified
• Test Specificity: This rabbit polyclonal antibody detects GABA A receptor alpha 6 subunit. It targets an epitope with in 16 amino acids from the N-terminal region.