GAPDH Antibody (13H12) - Azide and BSA Free, Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP280749

Catalog No. NB013822

Description

GAPDH Monoclonal specifically detects GAPDH in Human, Mouse, Rat, Drosophila, Primate, Monkey, Sheep samples. It is validated for Western Blot, Simple Western, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunocytochemistry.

Specifications

• Antigen: GAPDH
• Classification: Monoclonal
• Concentration: 0.5 mg/mL
• Dilution: Western Blot 0.25 - 1 ug/ml. Use reported by customer review, Simple Western : 1:25, Immunohistochemistry 5 ug/ml, Immunocytochemistry/Immunofluorescence : 1:10, Immunohistochemistry-Paraffin : 5 ug/ml, Immunocytochemistry
• Gene Alias: aging-associated gene 9 protein, EC 1.2.1, EC 1.2.1.12, EC 2.6.99.-, G3PD, GAPD, glyceraldehyde 3-phosphate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, MGC88685, Peptidyl-cysteine S-nitrosylase GAPDH
• Host Species: Mouse
• Purification Method: Protein G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Based upon 91% sequence similarity with immunogen, this antibody is predicted to react with Guinea Pig, Sheep, Squirrel, Porcine/Pig, Ferret, Canine/Dog/Cat, Bovine, Reptile/ Rattlesnake and several other species. Immunogen shows 82% similarity to Xenopus and Zebrafish. Rat, sheep, and monkey reactivity reported in scientific literature (PMID: 24796753, PMID: 27618403, and PMID: 24462973 respectively).
• Content And Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
• Isotype: IgG1 κ

• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin)
• Clone: 13H12
• Conjugate: Unconjugated
• Formulation: PBS
• Gene Symbols: GAPDH
• Immunogen: Amino acids between 275 and 325 of glyceraldehyde 3-phosphate dehydrogenase protein were used as the immunogen for this GAPDH antibody.
• Quantity: 0.1 mg
• Research Discipline: Alzheimers Research, Apoptosis, Autophagy, Cancer, Chromatin Research, DNA Repair, Lipid and Metabolism, Loading Controls, Neurodegeneration, Neuronal Cell Markers, Neuroscience, Nuclear Receptors Coactivators and Corepressors, Signal Transduction, Translation Control
• Gene ID (Entrez): 2597
• Target Species: Human, Mouse, Rat, Drosophila, Primate, Monkey, Sheep
• Form: Purified