GAPDH Antibody (1D4), Novus Biologicals™

Mouse Monoclonal Antibody has been used in 334 publications

Supplier:  Novus Biologicals NB300221

Catalog No. NB300221

Description

GAPDH Monoclonal specifically detects GAPDH in Human, Mouse, Rat, Porcine, Avian, Bovine, Canine, Chicken, Chinese Hamster, Drosophila, Equine, Feline, Hamster, Opossum, Primate, Rabbit, Sheep, Zebrafish samples. It is validated for Western Blot, Simple Western, Chromatin Immunoprecipitation, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Frozen, Single Cell Western, Knockdown Validated.

Specifications

• Antigen: GAPDH
• Classification: Monoclonal
• Concentration: 1.0 mg/mL
• Dilution: Western Blot 1:1000, Simple Western 1:25, Immunohistochemistry 1:100, Immunocytochemistry/Immunofluorescence 1:100, Immunoprecipitation, Immunohistochemistry-Frozen, Chromatin Immunoprecipitation (ChIP) 1:10-1:500, Single Cell Western, KnockDown Validated
• Gene Accession No.: P00355
• Gene Symbols: GAPDH
• Immunogen: This GAPDH antibody was developed against full length purified porcine GAPDH [UniProt# P00355]
• Purification Method: Immunogen affinity purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Human, mouse, rat, bovine, porcine, avian, sheep, opossum and primate. Likely to react with other mammals as well. Hamster and zebrafish reactivity reported by a customer review. Chicken reactivity reported in scientific literature (PMID: 24725420).
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Isotype: IgM

• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
• Clone: 1D4
• Conjugate: Unconjugated
• Formulation: 50% PBS, 50% Glycerol with 5mM Sodium Azide
• Gene Alias: 1D4 GAPDH, aging-associated gene 9 protein, Cytoplasm Marker, EC 1.2.1, EC 1.2.1.12, EC 2.6.99.-, G3PD, GAPD, GAPDPeptidyl-cysteine S-nitrosylase GAPDH, glyceraldehyde 3-phosphate dehydrogenase, glyceraldehyde-3-phosphate dehydrogenase, MGC102544, MGC102546, MGC103190, MGC103191, MGC105239, MGC127711, MGC88685, OCAS, p38, OCT1 coactivator in S phase, Peptidyl-cysteine S-nitrosylase GAPDH
• Host Species: Mouse
• Molecular Weight of Antigen: 36 kDa
• Quantity: 0.1 mL
• Research Discipline: Alzheimers Research, Apoptosis, Autophagy, Cancer, DNA Repair, Lipid and Metabolism, Loading Controls, Neurodegeneration, Neuronal Cell Markers, Neuroscience, Nuclear Receptors Coactivators and Corepressors, Signal Transduction, Translation Control
• Gene ID (Entrez): 2597
• Target Species: Human, Mouse, Rat, Pig, Avian, Bovine, Canine, Chicken, Chinese Hamster, Drosophila, Equine, Feline, Hamster, Opossum, Primate, Rabbit, Sheep, Zebrafish
• Form: Supernatant

For Research Use Only