GDF3 Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Rabbit Polyclonal Antibody

Catalog Number	Mfr. No.	Quantity
500P235BT25	Invitrogen™ 500P235BT25UG	25 μg
500P235BT50	Invitrogen™ 500P235BT50UG	50 μg
50-271-8586	Invitrogen™ 500P235BT500UG	500 μg

Description

AA Sequence of recombinant protein: AAIPVPKLSC KNLCHRHQLF INFRDLGWHK WIIAPKGFMA NYCHGECPFS LTISLNSSNY AFMQALMHAV DPEIPQAVCI PTKLSPISML YQDNNDNVIL RHYEDMVVDE CGCG. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human GDF-3. Anti-Human GDF-3-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect hGDF-3 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human GDF-3 (500-P235) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hGDF-3. Western Blot: To detect hGDF-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hGDF-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P235BT-1MG will be provided as 2 x 500 μg

GDF3 is a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site which is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. The function of this protein is unknown, but expression studies suggest it may be involved in regulation of the adult lymphatic and erythroid systems and embryonic development.

Specifications

• Antigen: GDF3
• Applications: ELISA, Western Blot
• Conjugate: Biotin
• Host Species: Rabbit
• Target Species: Human
• Gene Accession No.: Q9NR23
• Gene ID (Entrez): 9573
• Immunogen: E.coli-derived Recombinant Human GDF-3.
• Purification Method: Antigen affinity chromatography
• Gene: GDF3

• Concentration: 0.1-1.0 mg/mL
• Classification: Polyclonal
• Form: Lyophilized
• Regulatory Status: RUO
• Formulation: PBS with no preservative
• Gene Alias: C78318; ecat9; Gdf3; Gdf-3; growth differentiation factor 3; growth/differentiation factor 3; KFS3; MCOP7; MCOPCB6; RGD1564178; UNQ222/PRO248; VG-1-related protein 2; Vgr2; Vgr-2
• Gene Symbols: GDF3
• Primary or Secondary: Primary
• Content And Storage: -20°C