GE Healthcare™ Ficoll PM400 Available on GSA/VA Contract for Federal Government customers only.

High molecular weight sucrose-polymers formed by copolymerization of sucrose with epichlorohydrin. GE Healthcare™ Ficoll PM400 is a synthetic neutral, highly-branched hydrophilic polymer of sucrose, used for producing density gradients for separation of cells and subcellular components by centrifugation or by sedimentation at unit gravity.

Manufacturer: GE Healthcare™ 17-0300-05

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Catalog No. 45-001-792

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    Quantity 5kg
    Density 1.2g/mL
    For Use With (Application) Produces density gradients to separate cells, subcellular components
    Format Powder
    Viscosity ≈17mL/g
    Endotoxin Level 0.12EU/mg
    Product Type Centrifugation Media
    Shelf Life 5 years
    Storage Requirements 4° to 30°C
    dimDensity 1.2g/mL

    • For producing density gradients for separation of cells and subcellular components by centrifugation or by sedimentation at unit gravity
    • Neutral, highly branched, hydrophilic polymer of sucrose which dissolves readily in aqueous solution
    • Concentrations of up to 50% (w/v) covering densities of up to 1.2g/mL can be achieved
    • Has better osmotic properties than sucrose
    • Preserves functional and morphological integrity
    • Useful for separating cells that are sensitive to centrifugation and for separating cells of similar density but different size
    • Does not penetrate biological membranes
    • Serves as the raw material for preparation of Ficoll-Paque gradients
    • Used in other applications such as electrophoresis, hybridization, cryopreservation, and as a hapten carrier
    • Supplied as dry powders

    • Used in the formation of density gradients for the separation and isolation of eukaryotic cells, organelles, and bacterial cells, as a stabilizing agent and as a separation medium for the isolation of lymphocytes
    • Used in defined culture media, nucleic acid hybridization, electrophoresis, and immunological studies
    • Used for gradient centrifugation in all types of centrifuge rotors and for separation at unit gravity

  • Description & Specifications

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