For producing density gradients for separation of cells and subcellular components by centrifugation or by sedimentation at unit gravity
Neutral, highly branched, hydrophilic polymer of sucrose which dissolves readily in aqueous solution
Concentrations of up to 50% (w/v) covering densities of up to 1.2g/mL can be achieved
Has better osmotic properties than sucrose
Preserves functional and morphological integrity
Useful for separating cells that are sensitive to centrifugation and for separating cells of similar density but different size
Does not penetrate biological membranes
Serves as the raw material for preparation of Ficoll-Paque gradients
Used in other applications such as electrophoresis, hybridization, cryopreservation, and as a hapten carrier
Supplied as dry powders
Used in the formation of density gradients for the separation and isolation of eukaryotic cells, organelles, and bacterial cells, as a stabilizing agent and as a separation medium for the isolation of lymphocytes
Used in defined culture media, nucleic acid hybridization, electrophoresis, and immunological studies
Used for gradient centrifugation in all types of centrifuge rotors and for separation at unit gravity
Produces density gradients to separate cells, subcellular components
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