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GE Healthcare Glutathione Sepharose™ 4 Fast Flow Media

Single-step purification of GST-tagged proteins and other glutathione S-transferase or glutathione-dependent proteins. Glutathione Sepharose 4 Fast Flow Media is used for the purification of GST-tagged proteins from bacterial lysates and other S-tranferases or glutathione-dependent proteins.

Manufacturer:  GE Healthcare 17513202

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Catalog No. 45-000-286


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Description

Description

  • Glutathione coupled to Sepharose 4 Fast Flow via 10-carbon spacer arm
  • Protein binding capacity: »10mg recombinant GST/mL media (flowrate dependent)
  • Binding capacity and high flow properties allow scale-up
  • Mild elution conditions preserve protein antigenicity and function
  • Stable in all commonly used aqueous buffers
  • pH stability: 3 to 12

Specifications

Specifications

>10 mg/mL
<450cm/hr.
Glutathione and 10-carbon linker arm
100 mL
90μm
base matrix 150-250 cm/h, 100 kPa, XK 50/60 column, bed height 25 cm
4 to 30°C, 20% Ethanol
Glutathione Sepharose 4 Fast Flow is an affinity resin especially suited for purification of GST-tagged proteins in a batch mode or when scale up is needed.
10 Carbon spacer arm, 4% Agarose, 90μm particle size
Highly cross-linked 4% agarose
3 to 12
Glutathione Sepharose 4 Fast Flow, 100 mL
100mL
4% Highly Cross-linked Agarose
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