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GE Healthcare Glutathione Sepharose™ High Performance Media

For high resolution purification of Glutathione S transferase (GST) tagged proteins

Manufacturer:  GE Healthcare 17527901

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Catalog No. 45-000-330


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Description

Description

  • One-step purification of GST-tagged proteins and other glutathione S-transferases or glutathione-dependent proteins
  • GST-tagged proteins are eluted under mild, nondenaturing conditions using reduced glutathione
  • Mild purification process preserves protein antigenicity and function
  • Glutathione ligand is coupled to 6% agarose matrix

Specifications

Specifications

>Approx. 10 mg GST-tagged protein/mL resin
<600cm/hr.
Glutathione
25 mL
34μm
25mL
6% Highly Cross-linked Agarose
Glutathione Sepharose High Performance is an affinity resin recommended for purifying glutathione S-transferase (GST)-tagged proteins when high resolution is the priority.
Binding capacity 10mg recombinant GST/mL medium, Highly crosslinked Agarose, 6%
Highly crossed-linked agarose, 6%
3 to 12
Glutathione Sepharose High Performance, 25 mL
4 to 30°C, 20% Ethanol
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