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GE Healthcare GSTPrep™ FF 16/10 Column

Fast and easy one-step, scale-up purification of GST-tagged recombinant proteins

Manufacturer:  GE Healthcare 28936550

Catalog No. 45-002-074

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Glutathione Sepharose™ 4 Fast Flow is prepacked in 20mL GSTPrep columns for purification of GST-tagged proteins (as well as glutathione S-transferases and glutathione-dependent proteins). Mild elution conditions preserve protein antigenicity and function.


  • Binds approx. 200mg recombinant GST/column
  • Simple operation with pump or chromatography system (e.g., AKTA™)

GSTPrep FF 16/10 Prepacked Columns

  • Volume: 20mL
  • Dimensions: 1.6 x 10cm
  • Recommended flowrate (H2O at room temp): 1 to 10mL/min. (30 to 300cm/hr.)
  • Max. flowrate (H2O at room temp): 10mL/min. (300cm/hr.)
  • Max. pressure during operation (H2O at room temp): 1.5bar (0.15MPa, 22psi)
  • Hardware pressure limit: 5bar (0.5MPa, 73psi)
  • Storage/temperature: 20% ethanol/4° to 30°C



1M Acetate, pH 6, 0.1M NaOH, 70% Ethanol or 6M Guanidine Hydrochloride
0.62 in.
GSTPrep Glutathione Sepharose 4 FF column
Purification of Glutathione S-transferases and Glutathione-dependent Proteins
Glutathione and 10 Carbon linker arm
3 to 12
4% Highly Cross-linked Agarose
1 x 20mL
3.9 in.
1.5 bar
4° to 30°C
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