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Cytiva HiScreen™ Q Sepharose™ and SP Sepharose™ High Performance IEX Columns
Designed for method optimization and parameter screening
Manufacturer: Cytiva 28950511
Description
- Ready-to-use columns for convenient process development optimization and parameter screening
- Highly cross-linked agarose matrix with an average bead size of 34μm
- Bed height of 10cm designed to allow method optimization and parameter screening
- Easily connected in series to achieve 20cm bed height
- Small bed volume (4.7mL) gives fast result and minimal sample/buffer consumption
- Reproducible results, scalable to BioProcess columns packed with the same chromatography media using the same linear fluid velocity
- Bed dimension: 7.7 × 100mm
- Matrix: 6% cross-linked agarose beads
- Average particle size: 34μm
- Max. flow velocity: <150cm/hr.
- Material (column hardware and column tube): polypropylene
- Column I.D.: 7.7mm
- Flow velocity: <150cm/hr.
HiScreen Q HP
- Ionic capacity: 0.14 to 0.20mmol Cl-/mL
- Ligand: quaternary amine
- Ion exchanger type: strong anion exchanger
- Ionic capacity: 0.15 to 0.20mmol Cl-/mL
- Ligand: sulphopropyl
- Ion exchanger type: strong cation exchanger
Specifications
4.7mL | |
0.3 in. | |
<150cm/hr. | |
3.93 in. | |
Quaternary Amine | |
3 bar | |
HiScreen Q Sepharose HP column | |
4° to 30°C | |
2 to 12 |
8M Urea, 6M Guanidine HCl, 70% Ethanol, 1M NaOH, 1M Acetic Acid, 30% Isopropanol, 30% Aceto nitrile, 2% SDS | |
7.7mm | |
Optimization and parameter screening, ion exchange chromato graphy | |
100mm | |
Polypropylene | |
45 to 165μm | |
1 x 4.7mL | |
6% Cross-linked Agarose |
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