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Cytiva HiScreen™ Q Sepharose™ and SP Sepharose™ High Performance IEX Columns

Designed for method optimization and parameter screening

Manufacturer:  Cytiva 28950511

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Catalog No. 45-002-540

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  • Ready-to-use columns for convenient process development optimization and parameter screening
  • Highly cross-linked agarose matrix with an average bead size of 34μm
  • Bed height of 10cm designed to allow method optimization and parameter screening
  • Easily connected in series to achieve 20cm bed height
  • Small bed volume (4.7mL) gives fast result and minimal sample/buffer consumption
  • Reproducible results, scalable to BioProcess columns packed with the same chromatography media using the same linear fluid velocity

  • Bed dimension: 7.7 × 100mm
  • Matrix: 6% cross-linked agarose beads
  • Average particle size: 34μm
  • Max. flow velocity: <150cm/hr.
  • Material (column hardware and column tube): polypropylene
  • Column I.D.: 7.7mm
  • Flow velocity: <150cm/hr.

HiScreen Q HP

  • Ionic capacity: 0.14 to 0.20mmol Cl-/mL
  • Ligand: quaternary amine
  • Ion exchanger type: strong anion exchanger
HiScreen SP HP
  • Ionic capacity: 0.15 to 0.20mmol Cl-/mL
  • Ligand: sulphopropyl
  • Ion exchanger type: strong cation exchanger



0.3 in.
3.93 in.
Quaternary Amine
3 bar
HiScreen Q Sepharose HP column
4° to 30°C
2 to 12
8M Urea, 6M Guanidine HCl, 70% Ethanol, 1M NaOH, 1M Acetic Acid, 30% Isopropanol, 30% Aceto nitrile, 2% SDS
Optimization and parameter screening, ion exchange chromato graphy
45 to 165μm
1 x 4.7mL
6% Cross-linked Agarose
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