GE Healthcare HisTrap™ excel Prepacked Columns Available on GSA/VA Contract for Federal Government customers only.

For capture and purification of histidine-tagged proteins secreted into eukaryotic cell culture supernatants.   GE Healthcare Lifescience™ HisTrap™ excel Prepacked Columns are designed for fast and convenient purifications.

Manufacturer: GE Healthcare 29048586

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Catalog No. 45-000-505

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    Specifications

    Bed Volume 1mL
    Diameter (English) Inner 0.2 in.
    Diameter (Metric) Inner 7mm
    Flow Rate 1 to 4mL/min.
    Material Polypropylene
    Packaging Quantity 1 x 1mL
    pH Range 2 to 12
    Product Type HisTrap Excel Column
    Pressure 5 bar
    Quantity 1mL
    Storage Requirements 4° to 30°C
    Support Type 6% Highly Cross-linked Agarose

    The design of the columns in combination with the specific properties of the medium enables fast and convenient purifications.

    • Ready-to use prepacked with Ni Sepharose excel
    • Special type of filter in top and bottom of the columns to load large volumes of cell-free, un clarified samples directly on the columns helps prevent degradation and loss of sensitive target proteins
    • Made of biocompatible polypropylene and cannot be opened or refilled
    • Delivered with stopper on inlet and snap-off end on outlet
    • Operated either with peristaltic pump or with chromatography systems such as KTA systems
    • Binding Capacity/Column: At least 10 mg histidine-tagged protein/ml sedimented medium
    Media
    • Average particle size: 90µm
    • Matrix: Highly cross-linked agarose, 6%
    • pH Stability working range: 2 to 12
    • pH Stability cleaning range: 2 to 14
    • Flow velocity: 150 cm/h - 600 cm/h4
    Column:
    • Column I.D.: 7 mm

    HisTrap excel 1mL column

    • Bed Dimensions: 7 x 25mm
    • Flow rate: 1 to 4mL/min.
    HisTrap excel 5mL column
    • Bed Dimensions: 16 x 25mm
    • Flow rate: 5 to 20mL/min.

  • Description & Specifications

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