GE Healthcare HiTrap™ Protein A HP Prepacked Columns Available on GSA/VA Contract for Federal Government customers only.

Use for purification and isolation of monoclonal and polyclonal IgG from ascites, serum and cell culture supernatants. GE Healthcare Life Sciences™ HiTrap™ Protein A HP Prepacked Columns are specially designed, together with the medium to provide fast, simple and easy separations in a convenient format.

Manufacturer: GE Healthcare 17040203

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Catalog No. 45-000-050

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    Specifications

    Quantity 2 x 1mL
    Binding Capacity 20mg IgG/mL medium
    Flow Rate <4mL/min.
    For Use With (Application) For purification of monoclonal and polyclonal antibodies from ascites, serum and cell culture supernatants
    For Use With (Equipment) For syringe, peristaltic pump or a chromatography system
    Storage Requirements 4° to 30°C

    • Prepacked with Protein A Sepharose High Performance media for purification and fractionation of IgG subclasses
    • For use with syringe, pump or chromatography system such as KTA design or FPLC System
    • Binds 20mg human IgG/mL medium
    • Stable over pH 3 to 9 for use and 2 to 10 for cleaning
    • Designed for use with a syringe, a peristaltic pump, or a chromatography system
    • Maximum pressure: 3 bar
    • Column material: polypropylene
    • 1 and 5mL columns

    Media
    • Ligand: Native protein A
    • Matrix: 6% cross-linked agarose
    • Average particle size: 34µm
    • pH stability working range: 3 to 9

    1mL columns

    • Bed dimensions: 7 x 25mm
    • Flow velocity: <4mL/min.

    5mL columns
    • Bed dimensions: 16 x 25mm
    • Flow velocity: <20mL/min.

  • Description & Specifications

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