Cytiva (Formerly GE Healthcare Life Sciences) Protein G HP SpinTrap™ Columns 

For small-scale purification of antibodies or enrichment of proteins of interest in prepacked spin columns

Manufacturer:  Cytiva (Formerly GE Healthcare Life Sciences) 28903134

Catalog No. 45-001-485

Includes: 16 prepacked Protein G HP SpinTrap columns, one plastic bottom cap removal tool, and instructions. Also included is a protocol, with no pretreatment of sample required, for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min.

Description

• Protocols for both classic elution and cross-link methods; designed for use with a standard microcentrifuge
• Formatted for elution for both electrophoresis and LC-MS analysis workflows
• Fast-binding kinetics and high capacity provide high yield
• Prepacked with Protein G Sepharose™ High Performance Medium for coupling of antibodies of IgG subclasses
• Easy scale-up with HiTrap™ Protein G HP prepacked columns

• Matrix: Highly cross-linked agarose, 6%
• Binding capacity: >1mg human IgG/column
• Average particle size: 34µm
• pH stability: 3 to 9 (long term); 2 to 9 (short term)
• Column volume: 800µL
• Max. sample loading volume: 600µL

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Specifications

• Product Type: Protein G Sepharose HP SpinTrap spin column
• For Use With (Application): Small-scale purification of Antibodies or enrichment of Proteins of interest in prepacked spin column, electrophoresis or LC/MS
• Ligand Type: Recombinant Protein G lacking Albumin-binding region (2mg/mL medium)
• Particle Size: 34μm
• Storage Requirements: 4° to 8°C

• Quantity: 800μL
• Includes: 16 Prepacked Protein G HP SpinTrap column, one Plastic botto m cap removal to ol and instructions, proto col
• pH Range: 3 to 9 (long term), 2 to 9 (short term)
• Support Type: 6% Highly Cross-linked Agarose