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GE Healthcare Protein G HP SpinTrap™ Columns 
For small-scale purification of antibodies or enrichment of proteins of interest in prepacked spin columns
Manufacturer: GE Healthcare 28903134
Includes: 16 prepacked Protein G HP SpinTrap columns, one plastic bottom cap removal tool, and instructions. Also included is a protocol, with no pretreatment of sample required, for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min.
Description
- Protocols for both classic elution and cross-link methods; designed for use with a standard microcentrifuge
- Formatted for elution for both electrophoresis and LC-MS analysis workflows
- Fast-binding kinetics and high capacity provide high yield
- Prepacked with Protein G Sepharose™ High Performance Medium for coupling of antibodies of IgG subclasses
- Easy scale-up with HiTrap™ Protein G HP prepacked columns
- Matrix: Highly cross-linked agarose, 6%
- Binding capacity: >1mg human IgG/column
- Average particle size: 34µm
- pH stability: 3 to 9 (long term); 2 to 9 (short term)
- Column volume: 800µL
- Max. sample loading volume: 600µL
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Specifications
| Protein G Sepharose HP SpinTrap spin column | |
| Small-scale purification of Antibodies or enrichment of Proteins of interest in prepacked spin column, electrophoresis or LC/MS | |
| Recombinant Protein G lacking Albumin-binding region (2mg/mL medium) | |
| 34μm | |
| 4° to 8°C |
| 800μL | |
| 16 Prepacked Protein G HP SpinTrap column, one Plastic botto m cap removal to ol and instructions, proto col | |
| 3 to 9 (long term), 2 to 9 (short term) | |
| 6% Highly Cross-linked Agarose |
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