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GE Healthcare Ready-To-Go™ T4 DNA Ligase

For covalently linking DNA fragments in as little as 30 minutes

Manufacturer:  GE Healthcare 27036101

Catalog No. 45-001-317

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  • Catalyzes the formation of a phosphodiester bond between the 5'-phosphoryl group and the 3'-hydroxyl group of two double-stranded DNA fragments
  • Consists of a glassified reaction mix predispensed in single-reaction tubes
  • When reconstituted to final reaction volume of 20µL, yields solution of reaction buffer, T4 DNA ligase, and ATP



Ready-To-Go T4 DNA Ligase, 50 reaction beads
50 reactions
TE Buffer (10mM Tris HCl, pH 8, with 1mM EDTA) or 1-2X One-phor-all Buffer
FPLCpure™ T4 DNA Ligase 6 weiss units, 66mM Tris HCl (pH 7.6), 6.6mM MgCl2, 0.1mM ATP, 0.1mM Spermidine, 10mM DTT and stabilizer, glassified reaction mix predispensed in single-reaction tubes
Self-circularization of linear DNA prior to transformation, ligation of insert to vector and ligation of synthetic linkers to blunt-ended DNA
Room Temperature
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