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GE Healthcare rProtein A Sepharose™ Fast Flow Affinity Media

Designed for purification of monoclonal and polyclonal antibodies at labs and process scales

Manufacturer:  GE Healthcare 17127902

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Catalog No. 45-002-972

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  • Composed of recombinant protein A coupled with cross-linked 4% agarose base matrix
  • High dynamic binding capacity, single-point attachment of protein A, with high recovery, high purity and low ligand leakage
  • No mammalian components involved during manufacturing process
  • Matrix: 4% cross-linked agarose
  • Average particle size: 90µm (d50V)
  • Ligand: 6mg rProtein A/mL medium (E. coli)
  • Binding capacity: ∽50mg human IgG/mL media
  • pH stability working range: 3 to 10
  • Pressure/Flow specification: base matrix 150 to 250cm/hr., 100kPa, XK 50/60 column, bed


50 mg Human lgG/mL
For PurifiCatIon of Monoclonal and Polyclonal Antibodies at Labs and Process Scales
25 cm
4% Cross-Linked Agarose
25 mL
20% Ethanol
60 - 165 µm
2 to 9
150 to 250 cm/Hoµr
9.84 in.
Recombinant Protein A (E. coli)
100 kPa
Stable in aqueous Buffers commonly used in Protein A Chromatography
2°C to 8°C
rProtein A Sepharose fast flow media
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