GE Healthcare Sephacryl™ S-500 High Resolution Media Available on GSA/VA Contract for Federal Government customers only.

Hydrophilic, rigid allyl dextran/bisacrylamide matrix for fast and reproducible purification of proteins, polysaccharides and other macromolecules

Manufacturer: GE Healthcare 17061301

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Catalog No. 45-002-054

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    Quantity 750mL
    Storage Requirements 4° to 30°C
    Flow Rate >125cm/hr.
    Bed Volume 19 to 230L
    Product Type Sephacryl S-500 HR media
    Format Solid Spherical Bead
    Diameter (Metric) Inner 40 to 140cm
    For Use With (Application) Allow fast and reproducible purification of proteins, polysaccharides and other macromolecules by gel filtration at laborato ry and industrial scale
    Height (English) 5.9 in.
    Height (Metric) 15cm
    Max. Pressure 100kPa
    Particle Size 25 to 75μm
    pH Range 3 to 11
    Support Type Allyl Dextran and N, N'-methylenebisacrylamide
    Sample Type Polysaccharides, macromolecules and small plasmids

    Chromatography media offer good recovery and low nonspecific adsorption. Enable gel filtration at laboratory and industrial scale, and may be purchased in prepacked columns.

    • For separating polysaccharides, macromolecules with extended structures, and small plasmids
    • Excellent purification over a wide molecular weight range
    • High reproducibility due to long-term chemical and physical stability
    • High flow rates and recoveries
    • Well-suited to industrial-scale use
    • Storage conditions: 20% ethanol; 4° to 30°C
    • Available in a variety of selectivities and volumes

    Chemical Stability (stable in all common buffers):

    • 1M acetic acid, 8M urea
    • 6M guanidine-HCl, 1% SDS
    • 2M NaCl, 24% ethanol
    • 30% propanol, 30% acetonitrile
    • 0.5M NaOH (for cleaning)
    pH Stability
    • 3 to 11 (long-term and working)
    • 2 to 13 (short-term)
    Physical Stability:
    • Negligible volume variation due to changes in pH or ionic strength
    • Autoclavability: At 121°C, pH 7 for 30 minutes (gel only)
    • Antimicrobial agent: 20% ethanol

  • Description & Specifications

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