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GE Healthcare Sephadex™ G-25 Media

Use of Sephadex™ is  well established as a gel filtration medium for desalting and buffer exchange in industrial applications. GE Healthcare Life Sciences™ Sephadex™ G-25 Media quickly desalts, removes contaminants and transfers to a new buffer in a single step.

Manufacturer:  GE Healthcare 17003302

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Catalog No. 45-002-047

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Established medium for desalting and buffer exchange

  • Gel filtration medium
  • Available in coarse, medium, fine and superfine grades
  • All grades satisfy process-scale chromatography requirements
  • Available in lab packs or prepacked columns


  • Working pH range: 2 to 13
  • Bed volume (in distilled water): 4 to 6mL/g
  • Fractionation range dextrans/globular proteins: 1x 102 to 5 x 103 / 1 x 103 to 5 x 103 Mr
  • Max. flowrate/operating pressure: the beads behave as rigid spheres obeying Darcy's Law



Sephadex G-25 Medium, 500 g
11.81 in.
>100 cm/h, pressure drop cm H2O/bed height=2, bed height 30 cm, 2.6 cm i.d.
2 to 13
4 to 30°C, dry resins. Used resins 4 to 8 °C in 20% Ethanol or 0.01 M NaOH
4 to 6mL/g in Distilled Water
Solid Spherical Bead
Desalting and Buffer exchange and removal free label in industrial applications
38 to 235μm in wet form, >50μm in dry form
Cross-linked Dextran
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