GE Healthcare Sepharose™ Fast Flow Cation Exchange Media Available on GSA/VA Contract for Federal Government customers only.

Used for preparative protein separations in both research and industrial applications. GE Healthcare CM Sepharose™ Fast Flow Cation Exchange Media is composed of crosslinked 6% agarose beads, with carboxymethyl (CM) weak cation exchange groups.

Manufacturer: GE Healthcare 17072901

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Catalog No. 45-002-933

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    Stability Stable in commonly used aqueous Buffers: 1M NaOH, 8M Urea, 8M Guanidine Hydrochloride, 70% Ethanol
    Quantity 300mL
    Storage Requirements 4 to 30°C
    Anion Exchange Type Strong CatIon Exchanger
    Anion Exchange Capacity 0.18 to 0.25mM Hl+/mL
    Binding Capacity 70mg Ribonuclease A/mL medium
    Product Type SP Sepharose fast flow media
    For Use With (Application) For preparative Protein separations in research and industrial appliCatIons, for capture and intermediate purifiCatIon of wide range of approved biopharmaceuticals
    Flow Rate 400 to 700cm/Hour
    Particle Size 45 to 165μm
    Max. Pressure 100kPa
    Matrix 6% Cross-Linked Agarose
    pH Range 4 to 13
    Storage Buffer 20% Ethanol, 0.2M Sodium Acetate
    Ligand Type Sulphopropyl
    Packaging Quantity 300mL

    • Hydrophilic nature of base matrix ensures low levels of non-specific binding leading to low levels of host cell-derived impurities in the elution pool
    • Composed of crosslinked 6% agarose beads
    • Reproducible
    • Part of Sepharose Fast Flow ion exchange platform
    • High chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols
    • Available in range of pre-packed process development tools

    SP Sepharose:

    • Ligand: Sulphopropyl
    • Ion Exchanger Type: Strong cation exchanger
    • Ionic Capacity: 0.18 to 0.25mmol H+/mL medium
    • Binding capacity: 70mg ribonuclease A/mL medium
    CM Sepharose:
    • Ligand: Carboxymethyl
    • Ion Exchanger Type: Weak cation exchanger
    • Ionic Capacity: 0.09 to 0.13mmol Hl+/mL medium
    • Binding capacity: 50mg ribonuclease A/mL medium

    Used extensively for capture and intermediate purification of wide range of approved biopharmaceuticals

  • Description & Specifications

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