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GE Healthcare Sepharose™ Fast Flow Cation Exchange Media

SP Sepharose Fast Flow is a sulphopropyl (SP) strong cation exchange chromatography resin for fast protein purification. CM Sepharose Fast Flow is a weak cation exchanger based on the well established Sepharose Fast Flow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.

Manufacturer:  GE Healthcare 17071901

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Catalog No. 45-002-931


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Description

Description

SP Sepharose Fast Flow uses the Sepharose Fast Flow ion exchange resin, which is well established in industrial downstream processing. The highly crosslinked agarose resin enables high flow rates and high productivity.


CM Sepharose Fast Flow is a weak cation exchanger based on the well established Sepharose Fast Flow ion exchange platform, extensively used for preparative protein separations in both research and industrial applications.
 

  • Hydrophilic nature of base matrix ensures low levels of non-specific binding leading to low levels of host cell-derived impurities in the elution pool
  • Composed of crosslinked 6% agarose beads
  • Reproducible
  • Part of Sepharose Fast Flow ion exchange platform
  • High chemical stability, allowing well proven cleaning-in-place (CIP) and sanitization protocols
  • Available in range of pre-packed process development tools

SP Sepharose:

  • Ligand: Sulphopropyl
  • Ion Exchanger Type: Strong cation exchanger
  • Ionic Capacity: 0.18 to 0.25mmol H+/mL medium
  • Binding capacity: 70mg ribonuclease A/mL medium
CM Sepharose:
  • Ligand: Carboxymethyl
  • Ion Exchanger Type: Weak cation exchanger
  • Ionic Capacity: 0.09 to 0.13mmol Hl+/mL medium
  • Binding capacity: 50mg ribonuclease A/mL medium

Used extensively for capture and intermediate purification of wide range of approved biopharmaceuticals

Specifications

Specifications

0.09 to 0.13 mM Hl+/mL
50 mg Ribonuclease A/mL mediµm
For Preparative Protein Separations in Research and Industrial AppliCatIons, for Capture and Intermediate PurifiCatIon of Wide Range of Approved Biopharmaceuticals
6% Cross-Linked Agarose
500 mL
Stable in commonly used aqueous Buffers: 1 M NaOH, 8 M Urea, 8 M Guanidine Hydrochloride, 70% Ethanol
4°C to 30°C
CM Sepharose fast flow media
Weak CatIon Exchanger
300 to 600 cm/Hoµr
Carboxymethyl
100 kPa
500 mL
20% Ethanol
45 - 165 µm
4 to 13
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