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GE Healthcare Streptavidin HP MultiTrap™ Plates

Fast, reliable protein enrichment through immobilized biotinylated biomolecules in prepacked 96-well filter plates

Manufacturer:  GE Healthcare 28903131

Catalog No. 45-001-482


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Description

Description

  • Flexible protocol with elution conditions formatted for both electrophoresis and LC-MS analysis workflows
  • Useful for exploiting either the strong interactions of biotin and streptavidin or the somewhat weaker interactions of 2-iminobiotin and streptavidin
  • Can also be used for the direct enrichment of proteins that are biotinylated. By binding a specific biotinylated protein to the column, protein-protein interactions can also be investigated
  • Each well is prepacked with Streptavidin Sepharose™ High Performance Medium
  • Easy scale-up to Streptavidin HP SpinTrap™ or HiTrap™ Streptavidin HP prepacked columns
  • Can be used with centrifugation or vacuum, manually or in automated robotic systems

Streptavidin Sepharose HP Medium

  • Matrix: Highly cross-linked agarose, 6%
  • Binding capacity: >300nmol biotin/mL medium; 6mg biotinylated BSA/mL medium
  • Ave. particle size: 34µm
  • pH Stability (long-term): 4 to 9
Plates
  • Well volume: 800µL
  • Max. sample loading volume: 600µL
  • Medium volume: 50µL

Specifications

Specifications

>300nM/mL
Protein enrichment, Protein-Protein interaction investigations
Polypropylene, Polyethylene
Streptavidin HP MultiTrap Plate
4° to 8°C
6% Highly Cross-linked Agarose
<150cm/hr.
Streptavidin Sepharose HP, spin column and 96-well filter plates filter plates
34μm
4 x 96 Well Plate
4 x 96-well plate
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