Invitrogen™ Precision gRNA Synthesis Kit

Description

Includes

BOX 1

• 5X TranscriptAidTM Reaction Buffer
• DNase I, RNase-free (1 U/μL)
• Nuclease-free water
• NTP mix (100 mM each of ATP, GTP, CTP,
• UTP in Tris buffer)
• Control gRNA forward and reverse primers (10 μM mix) 2

BOX 2

• Binding Buffer
• Wash Buffer 1 (concentrated)3
• Wash Buffer 2 (concentrated)4
• Nuclease-free water
• Gene JETTM RNA Purification Micro Column & Collection Tubes
• Elution Tubes, 1.5 mL

Simple, ready-to-use, all-in-one expression vector system for CRISPR-Cas9. Transfection-ready guide RNA (gRNA) in as little as four hours.

Simple, ready-to-use, all-in-one expression vector system for CRISPR-Cas9. GeneArt Precision gRNA Synthesis Kit is a complete system for rapid synthesis of guide RNA (gRNA) ready to complex with GeneArt Platinum Cas9 Nuclease or GeneArt CRISPR Nuclease mRNA. Starting with two short single-stranded oligos that code for the target sequence, the gRNA template is assembled with a T7 promoter in a short one-pot PCR reaction. The assembled product is then used as template in an in vitro transcription (IVT) reaction followed by a rapid purification step, yielding transfection-ready gRNA in as little as four hours. Resulting gRNA can also be co-transfected with our ready-to-transfect GeneArt Platinum Cas9 Nuclease or GeneArt CRISPR Nuclease mRNA. Both protein and mRNA Cas9 formats require no plasmid manipulation and so are amenable to high throughput and multiplex genome-wide cell engineering approaches.

Features of the GeneArt Precision gRNA Synthesis Kit include:

• Fast assembly and synthesis of any gRNA target in as little as four hours including template assembly
• High yield (>10 ug) and concentration (>200 ng/uL) of gRNA

For research use only. Not for use in diagnostic procedures.

Certifications

CoA

Recommended Storage

gRNA Prep Kit, store at -5 to -30°C and gRNA Cleanup Kit, store at room temperature

Specifications

• Concentration: 200 ng/μL
• Content And Storage: • gRNA Prep Kit, store at -5°C to -30°C
  • - gRNA Cleanup Kit, store at room temperature (For better long-term performance, store purification columns at 2°C to 8°C)
• Form: Liquid
• Format: Kit
• Reaction Speed: Fast
• Technique: CRISPR-Cas9
• Product Type: gRNA Synthesis Kit
• Promoter: T7
• Quantity: 25 reactions
• Shipping Condition: Approved for shipment at Room Temperature or on Wet or Dry Ice
• Sufficient For: 25 Reactions
• Yield: >10 μg at >200 ng/μL of gRNA
• Final Product Type: RNA
• No. of Reactions: 25 Reactions
• Starting Material: Oligos (DNA)

For Research Use Only. Not for use in diagnostic procedures.