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Thermo Scientific™ GeneBLAzer™ CRHR2-CRE-bla CHO-K1 Cells

Catalog No. K1733
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Catalog No. K1733 Supplier Thermo Scientific™ Supplier No. K1733
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Description

GeneBLAzer™ CRHR2-CRE-bla CHO-K1 cells contain the human Corticotropin Releasing Factor Receptor 2 (CRHR2) stably integrated into the CellSensor™ CRE-bla CHO-K1 cell line.

GeneBLAzer™ CRHR2-CRE-bla CHO-K1 cells contain the human Corticotropin Releasing Factor Receptor 2 (CRHR2) stably integrated into the CellSensor™ CRE-bla CHO-K1 cell line. CellSensor™ CRE-bla CHO-K1 cells (Cat. No. K1535) contain a beta-lactamase reporter gene under control of the Cyclic AMP Response Element (CRE) response element.

The GeneBLAzer™ CRHR2-CRE-bla CHO-K1 cells are functionally validated for Z' and EC50 concentrations of CRF. In addition, GeneBLAzer™ CRHR2-CRE-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.

Corticotropin releasing factor is a 41-amino acid peptide that plays a role in the integration of autonomic, neuroendocrine, and behavioral responses to stress. These effects are mediated through two receptor families, CRHR1 and CRHR2. While CRF was originally isolated from the hypothalamus, where it was shown to be the primary neuroregulator mediating the hypothalamic-pituitary-adrenocortical stress axis, it has since been found to be widely distributed outside the hypothalamus throughout the central nervous system. Presently, there are five distinct targets for CRF with unique pharmacology and localization. These have been placed into three distinct classes, two of which are the G-protein-coupled receptors CRF1 (CRHR1) and CRF2 (CRHR2). Three functional splice variants have been identified for the mammalian CRHR2 receptor, although pharmacological characterization of these splice variants revealed no major differences between them.

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TRUSTED_SUSTAINABILITY

Specifications

Accession Number NM_001883.2
Assay Entry Cell-based beta lactamase reporter gene
Cell State Dividing Cells
Content And Storage LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.

Includes:
• GeneBLAzer™ CRHR2-CRE-bla CHO-K1 cells
• GeneBLAzer™ CRHR2-CRE-bla CHO-K1 Assay Protocol
• Certificate of Analysis

The GeneBLAzer™ CRHR2-CRE-bla CHO-K1 cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Detection Method Fluorescence
For Use With (Application) FRET
Gene Symbol CRHR2
Population Status Single Cell Clone Origin
Product Line GeneBLAzer
Quantity 2 x 106 Cells
Reporter Gene BLA (Beta-Lactamase)
Target Entry CRHR2
Cell Line CHO-K1
System Type GeneBLAzer™
Druggable Target GPCR
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For Research Use Only. Not for use in diagnostic procedures.

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