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Thermo Scientific™ GeneBLAzer™ D2-Gqo5-NFAT-bla CHO-K1 Cells

Catalog No. K1708
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Description

GeneBLAzer™ D2-Gqo5-NFAT-bla CHO-K1 cells contain the human Dopamine Receptor 2 (D2) stably integrated into the GeneBLAzer™ Gqo5-NFAT-bla CHO-K1 cell line.

For Academic pricing please login or contact us at discoverysciences@invitrogen.com.

GeneBLAzer™ D2-Gqo5-NFAT-bla CHO-K1 cells contain the human Dopamine Receptor 2 (D2) stably integrated into the GeneBLAzer™ Gqo5-NFAT-bla CHO-K1 cell line. GeneBLAzer™ Gqo5-NFAT-bla CHO-K1 cells (Cat. No. K1536) contain a beta-lactamase reporter gene under control of the Nuclear Factor of Activated T-cells (NFAT) response element and the Chimeric G protein, Gqo5.

The GeneBLAzer™ D2-Gqo5-NFAT-bla CHO-K1 cells are functionally validated for Z' and EC50 concentrations of apomorphine. In addition, GeneBLAzer™ D2-Gqo5-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.

The D2 receptor is found primarily in the brain. There are two forms of D2 which are created by alternate splicing: a short form and a long form. This assay uses the long form, although little difference in ligand binding or signaling has been seen between the two forms.

TRUSTED_SUSTAINABILITY

Specifications

Accession Number NM_000795
Assay Entry Cell-based beta lactamase reporter gene
Cell State Dividing Cells
Content And Storage LiveBLAzer™-FRET B/G Loading Kit, Solution D, and other materials are required separately; please refer to the protocol.

Includes:
• GeneBLAzer™ D2-Gqo5-NFAT-bla CHO-K1 cells
• GeneBLAzer™ D2-Gqo5-NFAT-bla CHO-K1 Assay Protocol
• Certificate of Analysis

The GeneBLAzer™ D2-Gqo5-NFAT-bla CHO-K1 cells should be stored in liquid nitrogen immediately upon receipt.
Detection Method Fluorescence
For Use With (Application) FRET
Gene Symbol D2
Population Status Single Cell Clone Origin
Product Line GeneBLAzer
Quantity 2 x 106 Cells
Reporter Gene BLA (Beta-Lactamase)
Target Entry DRD2, D2
Type CHO-K1 Cells
Cell Line CHO-K1
System Type GeneBLAzer™
Druggable Target GPCR
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For Research Use Only. Not for use in diagnostic procedures.

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