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Invitrogen™ GeneBLAzer™ ER Alpha DA Assay Kit

Catalog No. K1393
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K1393 1 plate
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Catalog No. K1393 Supplier Invitrogen™ Supplier No. K1393
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Description

GeneBLAzer™ ER Alpha DA Assay Kit

GeneBLAzer™ER alpha DA(Division Arrested) cells and ER alpha-UAS-bla GripTite™ cells contain the ligand-binding domain (LBD) of the human Estrogen receptor alpha (ER alpha) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla GripTite™ cell line. GeneBLAzer™UAS-bla GripTite™ cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-ER alpha (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both ER alpha DA cells and ER alpha-UAS-bla GripTite™cells are functionally validated for Z' and EC50 concentrations of 17-beta Estradiol. In addition, ER alpha-UAS-bla GripTite™cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.
TRUSTED_SUSTAINABILITY

Specifications

Alternate Agonists Betamethasone, Cortisol, Budesonide
Antagonists Milfepristone, Cortisone, Progesterone
Assay Entry Cell-based beta lactamase reporter gene
Cell State Division-Arrested Cells
Content And Storage Shipping: Dry IceStorage: -80°C/Liquid NitrogenSufficient division-arrested cells and substrate to assay one 384-well plate. Includes:ER alpha GripTite™ DA cells (K1391A)LiveBLAzer™-FRET B/G Loading Kit, 70 μg
Detection Method Fluorescence
Gene ID (Entrez) 2099
Gene Symbol ERα
Primary Agonist Dexamethasone
Product Line GeneBLAzer
Quantity 1 plate
Reporter Gene BLA (Beta-Lactamase)
Target Entry ER alpha, ESR1, ERA, NR3A1
Type Division-Arrested Cells Assay Kit
Cell Line 293T (HEK)
System Type GeneBLAzer™
Druggable Target Nuclear Receptors
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For Research Use Only. Not for use in diagnostic procedures.

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