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Thermo Scientific™ GeneBLAzer™ Gα15-NFAT CHO-K1 DA Assay Kit

Catalog No. K1453
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Catalog No. K1453 Supplier Thermo Scientific™ Supplier No. K1453
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Description

The GeneBLAzer™ Gα15-NFAT CHO-K1 DA (Division Arrested) cell line contains the promiscuous G protein, Gα15, stably integrated into the CellSensor™ NFAT-bla CHO-K1 cell line.

The GeneBLAzer™ Gα15-NFAT CHO-K1 DA (Division Arrested) cell line contains the promiscuous G protein, Gα15, stably integrated into the CellSensor™ NFAT-bla CHO-K1 (Cat. No. K1078) cell line. NFAT-bla CHO-K1 cells contain a beta-lactamase reporter gene under control of an NFAT response element stably integrated into CHO-K1 cells. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.

This product can serve as a negative control in screening assays performed with specific G-protein coupled receptor (GPCR) DA cells that were made from this same background. These cells can also be used in other assays where a sensitive readout to intracellular changes in calcium is needed.

TRUSTED_SUSTAINABILITY

Specifications

Cell State Division-Arrested Cells
Content And Storage Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384-well plate. (Other materials are required separately; please refer to the protocol).

Includes:
• GeneBLAzer™ Gα15-NFAT CHO-K1 DA cells
• LiveBLAzer™-FRET B/G Loading Kit, 70 μg
• Solution D
• GeneBLAzer™ Gα15-NFAT CHO-K1 Assay Protocol
• LiveBLAzer™-FRET B/G Loading Protocol
• Certificate of Analysis

The GeneBLAzer™ Gα15-NFAT CHO-K1 DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Detection Method Fluorescence
Gene Symbol Gα15
Product Line GeneBLAzer
Quantity 1 Plate
Reporter Gene BLA (Beta-Lactamase)
Cell Line CHO-K1
System Type GeneBLAzer™
Druggable Target GPCR

For Research Use Only. Not for use in diagnostic procedures.

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