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Thermo Scientific™ GeneBLAzer™ Gqo5-NFAT-bla FreeStyle™ 293F Cells
Description
GeneBLAzer™ Gqo5-NFAT-bla FreeStyle™ 293F cells contain the chimeric G-protein Gqo5 stably integrated into the CellSensor™ NFAT-blaFreeStyle™ 293F cell line. CellSensor™ NFAT-bla FreeStyle™ 293F cells contain a beta-lactamase reporter gene under control of a stably integrated NFAT response element. Gqo5-NFAT-bla FreeStyle™ 293F is a parental cell line designed to be used for the construction of Gi/o-coupled GPCR beta-lactamase cellular assays. The Gi/o receptor of interest should be stably expressed in the cell line by expression from the selectable vector pcDNA™6.2-hygro-DEST (Cat. No. K1233). Gqo5-NFAT-bla FreeStyle™ 293F cells have been shown to respond to the agonist of a transiently expressed D2 receptor.
Specifications
Specifications
| Cell State | Dividing Cells |
| Content And Storage | Shipped on dry ice. Store in liquid nitrogen immediately upon receipt or thaw for immediate use. |
| Detection Method | Fluorescence |
| Gene Symbol | Gqo5 |
| Product Line | GeneBLAzer |
| Quantity | 2 x 106 Cells |
| Reporter Gene | BLA (Beta-Lactamase) |
| Cell Line | FreeStyle 293-F Cells |
| System Type | GeneBLAzer™ |
| Druggable Target | GPCR |
For Research Use Only. Not for use in diagnostic procedures.
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