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Thermo Scientific™ GeneBLAzer™ HCRTR2-Gα15-NFAT-bla CHO-K1 Cells

Catalog No. K1737
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K1737 2 x 106 Cells
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Catalog No. K1737 Supplier Thermo Scientific™ Supplier No. K1737
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Description

GeneBLAzer™ HCRTR2-Gα15-NFAT-bla CHO-K1 cells contain the human Orexin Receptor B (HCRTR2) stably integrated into the GeneBLAzer™ Gα15-NFAT-bla CHO-K1 cell line.

GeneBLAzer™ HCRTR2-Gα15-NFAT-bla CHO-K1 cells contain the human Orexin Receptor B (HCRTR2) stably integrated into the GeneBLAzer™ Gα15-NFAT-bla CHO-K1 cell line. GeneBLAzer™ Gα15-NFAT-bla CHO-K1 cells (Cat. No. K1537) contain a beta-lactamase reporter gene under control of the Nuclear Factor of Activated T-cells (NFAT) response element and the Promiscuous G protein, Gα15.

The GeneBLAzer™ HCRTR2-Gα15-NFAT-bla CHO-K1 cells are functionally validated for Z' and EC50 concentrations of Orexin B. In addition, GeneBLAzer™ HCRTR2-Gα15-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary.

The HCRTR2 receptor has been detected in the brain as well as adrenal gland, gastrointestinal tract, and pancreas. The signaling cascades used by orexin receptors appear to be varied and cell type dependant. In CHO cells, calcium release is seen after agonist treatment. In hypothalamic neurons, the frequency of action potentials is increased. Administering either orexin peptide into the bloodstream of rats caused an increase in feeding behavior. The orexin system is also thought to regulate neuroendocrine function and the sleep wake cycle. HCRTR2 has been directly implicated in narcolepsy in animal studies.

For Academic pricing please login or contact us at discoverysciences@invitrogen.com.

TRUSTED_SUSTAINABILITY

Specifications

Accession Number NM_001526.2
Assay Entry Cell-based beta lactamase reporter gene
Cell State Dividing Cells
Content And Storage LiveBLAzer™-FRET B/G Loading Kit, Solution D and other materials are required separately; please refer to the protocol.

Includes:
• GeneBLAzer™ HCRTR2-Gα15-NFAT-bla CHO-K1 cells
• GeneBLAzer™ HCRTR2-Gα15-NFAT-bla CHO-K1 Assay Protocol
• Certificate of Analysis

The GeneBLAzer™ HCRTR2-Gα15-NFAT-bla CHO-K1 cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.
Detection Method Fluorescence
For Use With (Application) FRET
Gene Symbol HCRTR2
Population Status Single Cell Clone Origin
Product Line GeneBLAzer
Quantity 2 x 106 Cells
Reporter Gene BLA (Beta-Lactamase)
Target Entry HCRTR2
Cell Line CHO-K1
System Type GeneBLAzer™
Druggable Target GPCR
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For Research Use Only. Not for use in diagnostic procedures.

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