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Thermo Scientific™ GeneBLAzer™ MR DA Assay Kit

Catalog No. K1409
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K1409 1 plate
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Catalog No. K1409 Supplier Thermo Scientific™ Supplier No. K1409
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Description

GeneBLAzer™MR DA(Division Arrested) cells and MR-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Mineralcorticoid Receptor (MR) fused to the NAbinding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line.

GeneBLAzer™MR DA(Division Arrested) cells and MR-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Mineralcorticoid Receptor (MR) fused to the NAbinding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line. GeneBLAzer™UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-MR (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384- well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both MR DA cells and MRUAS-bla HEK 293T cells are functionally validated for Z' and EC50 concentrations of aldosterone. In addition, MR-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, and substrate loading time. Additional testing data using alternate stimuli are also available.

TRUSTED_SUSTAINABILITY

Specifications

Alternate Agonists AM 580, Fenretinide, 9-cis- retinoic acid, Ch 55, TTNPB
Antagonists LE 135
Assay Entry Cell-based beta lactamase reporter gene
Cell State Division-Arrested Cells
Content And Storage Contains sufficient division-arrested cells and substrate to assay one 384-well plate
• MR HEK 293T DA cells (K1409A)
• LiveBLAzer™-FRET B/G Loading Kit, 70 μg

Shipping: Dry Ice
Storage: -80°C (liquid nitrogen)
Detection Method Fluorescence
Gene Symbol MR
Primary Agonist All-trans-retinoic Acid
Product Line GeneBLAzer
Quantity 1 plate
Reporter Gene BLA (Beta-Lactamase)
Target Entry NR3C2, MR
Type Division-Arrested Cells Assay Kit
Cell Line 293T (HEK)
System Type GeneBLAzer™
Druggable Target Nuclear Receptors
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For Research Use Only. Not for use in diagnostic procedures.

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