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Thermo Scientific™ GeneBLAzer™ RAR-gamma DA Assay Kit

Catalog No. K1559
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K1559 1 Plate
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Catalog No. K1559 Supplier Thermo Scientific™ Supplier No. K1559
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Description

GeneBLAzer™RAR gamma DA (Division Arrested) cells and RAR gamma-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human retinoic acid receptor gamma (RAR gamma) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line.

GeneBLAzer™RAR gamma DA (Division Arrested) cells and RAR gamma-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human retinoic acid receptor gamma (RAR gamma) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line. GeneBLAzer™UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-RAR gamma (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both RAR gamma DA cells and RAR gamma-UAS-bla HEK 293T cells are functionally validated for Z' and EC50 concentrations of all-trans retinoic acid (ATRA). In addition, RAR gamma-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, and stimulation time.

TRUSTED_SUSTAINABILITY

Specifications

Alternate Agonists 4-hydroxytamoxifen, Chlordane, Toxaphene
Assay Entry Cell-based beta lactamase reporter gene
Cell State Division-Arrested Cells
Content And Storage Contains sufficient division-arrested cells and substrate to assay 1 x 384-well plate.
• RAR gamma HEK 293T DA cells (K1559A)
• LiveBLAzer™-FRET B/G Loading Kit, 70 μg

Shipping: Dry Ice
Storage: -80°C (liquid nitrogen)
Detection Method Fluorescence
Gene Symbol RARγ
Primary Agonist XCT790
Quantity 1 Plate
Reporter Gene BLA (Beta-Lactamase)
Target Entry RARG, RAR gamma, NR1B3
Type Division-Arrested Cells Assay Kit
Cell Line 293T (HEK)
System Type GeneBLAzer™
Druggable Target Nuclear Receptors
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For Research Use Only. Not for use in diagnostic procedures.

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