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Thermo Scientific™ GeneBLAzer™ RXR alpha DA Assay Kit

Catalog No. K1411
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K1411 1 plate
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Catalog No. K1411 Supplier Thermo Scientific™ Supplier No. K1411
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Description

GeneBLAzer™ RXR alpha DA (Division Arrested) cells and RXR alpha-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human retinoid X receptor-alpha (RXR alpha) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™ UASbla HEK 293T cell line.

GeneBLAzer™ RXR alpha DA (Division Arrested) cells and RXR alpha-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human retinoid X receptor-alpha (RXR alpha) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™ UASbla HEK 293T cell line. GeneBLAzer™ UAS-bla HEK 293T cells stably express a betalactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-RXR alpha (LBD) fusion protein, the protein binds to the UAS, resulting in expression of alpha-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both RXR alpha DA cells and RXR alpha-UAS-bla HEK 293T cells are functionally validated for Z' and EC50 concentrations of 9-cis-retinoic acid. In addition, RXR alpha-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.

TRUSTED_SUSTAINABILITY

Specifications

Alternate Agonists Dexamethasone, Progesterone, Cortisol
Antagonists Spironolactone
Assay Entry Cell-based beta lactamase reporter gene
Cell State Division-Arrested Cells
Content And Storage Contains sufficient division-arrested cells and substrate to assay 1 x 384-well plate:
• RXR alpha HEK 293T DA cells (K1411A)
• LiveBLAzer™-FRET B/G Loading Kit, 70 μg

Shipping: Dry Ice
Storage: -80°C (liquid nitrogen)
Detection Method Fluorescence
Gene Symbol RXRα
Primary Agonist Aldosterone
Product Line GeneBLAzer
Quantity 1 plate
Reporter Gene BLA (Beta-Lactamase)
Target Entry RXRA, RXR alpha, NR2B1
Type Division-Arrested Cells Assay Kit
Cell Line 293T (HEK)
System Type GeneBLAzer™
Druggable Target Nuclear Receptors
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For Research Use Only. Not for use in diagnostic procedures.

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