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Thermo Scientific™ GeneBLAzer™ TRα-UAS-bla HEK 293T Assay Kit

Catalog No. K1684
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K1684 1 Plate
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Catalog No. K1684 Supplier Thermo Scientific™ Supplier No. K1684

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Description

TR alpha-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Thyroid hormone receptor alpha(TR alpha) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line.

TR alpha-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Thyroid hormone receptor alpha(TR alpha) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line. GeneBLAzer™UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-TR alpha (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. TR alpha-UASbla HEK 293T 293 cells are functionally validated for Z' and EC50 concentrations of T3 Thryoid hormone. In addition, TR alpha-UAS-bla HEK 293T 293 cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.

TRUSTED_SUSTAINABILITY

Specifications

Alternate Agonists 9-cis-retinoic acid, TTNPB, CD437, AM 580
Antagonists AGN 193109
Assay Entry Cell-based beta lactamase reporter gene
Cell State Dividing Cells
Content And Storage TR alpha-UAS-bla HEK 293T cells (K1260)

Shipping: Dry Ice
Storage:-80°C (liquid nitrogen)
Detection Method Fluorescence
Primary Agonist All-trans-retinoic Acid
Product Line GeneBLAzer
Quantity 1 Plate
Reporter Gene BLA (Beta-Lactamase)
Target Entry THRA, TR alpha, NR1A1
Type Detection Assay Kit
Cell Line HEK 293T
System Type GeneBLAzer™
Druggable Target Nuclear Receptors
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For Research Use Only. Not for use in diagnostic procedures.

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