GFP Antibody (9F9.F9), Novus Biologicals™

Mouse Monoclonal Antibody has been used in 44 publications

Supplier:  Novus Biologicals NB600597SS

Catalog No. NB600597SS

Description

GFP Monoclonal specifically detects GFP in Non-species specific samples. It is validated for Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunoblotting, In vitro assay, Dot Blot, Knockdown Validated.

Specifications

• Antigen: GFP
• Classification: Monoclonal
• Concentration: 1 mg/ml
• Dilution: Western Blot 1:3000-1:30000, Flow Cytometry 1:1:10 - 1:1000, ELISA 1:10000-1:30000, Immunohistochemistry 1:1000-1:5000, Immunocytochemistry/Immunofluorescence 1:10 - 1:500, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:10 - 1:500, Immunoblotting, In vitro assay, Dot Blot 1:100 - 1:2000, Knockdown Validated
• Gene Alias: clone 9F9.F9, Enhanced Green Fluorescent Protein, GFP monoclonal, Green Fluorescent Protein, green fluorescent protein (gfp)
• Host Species: Mouse
• Molecular Weight of Antigen: 27 kDa
• Quantity: 0.025 mg
• Research Discipline: Cellular Markers, Epitope Tags, Fusion Proteins, Mitochondrial Fusion Proteins, Mitochondrial Markers
• Test Specificity: Reactivity is observed against recombinant Green Fluorescent Protein from Aequorea victoria by both Western blot and ELISA. No reaction is seen against RFP.
• Content And Storage: Store at -20°C. Avoid freeze-thaw cycles.
• Isotype: IgG1 κ

• Applications: Western Blot, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunoblot, In vitro Assay, Dot Blot, KnockDown
• Clone: 9F9.F9
• Conjugate: Unconjugated
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% Sodium Azide
• Gene Symbols: GFP
• Immunogen: Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria.
• Purification Method: Protein A purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Target Species: Jellyfish
• Form: Purified