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GFP Antibody, R&D Systems™
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Catalog No. p-7066001
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25 μg
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MAB42401SP 25 μg
MAB42401 100 μg
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Mouse Monoclonal Antibody

GFP Monoclonal specifically detects GFP in Multi-Species samples. It is validated for Immunoprecipitation, Intracellular Staining by Flow Cytometry, Immunocytochemistry, CyTOF-ready.

Specifications

Antigen GFP
Applications Immunoprecipitation, Flow Cytometry, Immunocytochemistry, CyTOF
Classification Monoclonal
Clone 454505
Concentration LYOPH
Conjugate Unconjugated
Dilution Immunoprecipitation 2 ug/100 ug cell lysate, Intracellular Staining by Flow Cytometry 0.25 ug/10^6 cells, Immunocytochemistry 8-25 ug/mL, CyTOF-ready
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS. with No Preservative
Gene Accession No. P42212
Gene Alias eGFP, Enhanced Green Fluorescent Protein, GFPuv, Green Fluorescent Protein, green fluorescent protein (gfp)
Gene Symbols GFP
Host Species Mouse
Immunogen E. coli-derived recombinant GFPuv Ser2-Lys238 Accession # P42212
Purification Method Protein A or G purified from hybridoma culture supernatant
Quantity 25 μg
Regulatory Status RUO
Primary or Secondary Primary
Test Specificity Stains eGFP transfectants but not irrelevant transfectants in flow cytometry. Detects recombinant GFPuv in Western blots. For optimal Western blot results, we recommend R&D Systems Goat Anti-GFP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4240 ) or Novus Biologicals Mouse Anti-GFP Monoclonal Antibody (Catalog # NBP2-2211 ).
Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.
Target Species Multi-species
Content And Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Form Purified
Isotype IgG1
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