Glypican 3 Antibody (GPC3/863), Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP2444860.02MG

Catalog No. NBP24448600

Description

Ensure accurate, reproducible results in Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence

Glypican 3 Monoclonal specifically detects Glypican 3 in Human, Rat samples. It is validated for Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunofluorescence.

Specifications

• Antigen: Glypican 3
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Dilution: Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml
• Gene Accession No.: P51654
• Gene Symbols: GPC3
• Immunogen: Recombinant full-length human GPC3 protein
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Gene ID (Entrez): 2719
• Target Species: Human, Rat
• Form: Purified

• Applications: Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence
• Clone: GPC3/863
• Conjugate: Unconjugated
• Formulation: 1.0mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene Alias: DGSX, glypican 3, glypican proteoglycan 3, glypican-3, GTR2-2, heparan sulphate proteoglycan, Intestinal protein OCI-5, MXR7, OCI5, OCI-5, secreted glypican-3, SGB, SGBS, SGBS1SDYS
• Host Species: Mouse
• Molecular Weight of Antigen: 67 kDa
• Quantity: 0.02 mg
• Primary or Secondary: Primary
• Test Specificity: Glypican-3 (GPC3) is a glycosylphospatidyl inositol-anchored membrane protein, which may also be found in a secreted form. Anti-GPC3 has been identified as a useful tumor marker for the diagnosis of hepatocellular carcinoma (HCC), hepatoblastoma, melanoma, testicular germ cell tumors, and Wilm s tumor. In patients with HCC, GPC3 is overexpressed in neoplastic liver tissue and elevated in serum, but is undetectable in normal liver, benign liver, and the serum of healthy donors. GPC3 expression is also found to be higher in HCC liver tissue than in cirrhotic liver or liver with focal lesions such as dysplastic nodules and areas of hepatic adenoma (HA) with malignant transformation. In the context of testicular germ cell tumors, GPC3 expression is up regulated in certain histologic subtypes, specifically yolk sac tumors and choriocarcinoma. A high level of GPC3 expression is also found in some types of embryonal tumors, such as Wilm s tumor and hepatoblastoma, with a low or undetectable expression in normal adjacent tissue. In patients with thyroid cancer, expression of GPC3 is dramatically enhanced in certain types of cancers: 100% in follicular carcinoma and 70% in papillary carcinoma. Expression of GPC3 in follicular carcinoma is significantly higher than that of follicular adenoma. In contrast, GPC3 is not expressed in anaplastic carcinoma.
• Content And Storage: Store at 4C.
• Isotype: IgG1 κ

For Research Use Only