Golgi Glycoprotein 1/GLG1 Antibody (GLG1/970), Novus Biologicals™

Mouse Monoclonal Antibody

Supplier:  Novus Biologicals NBP2443610.02MG

Catalog No. NBP24436100

Description

Ensure accurate, reproducible results in Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunocytochemistry, Immunofluorescence

Golgi Glycoprotein 1/GLG1 Monoclonal specifically detects Golgi Glycoprotein 1/GLG1 in Human, Mouse (Negative), Rat (Negative) samples. It is validated for Immunohistochemistry, Immunohistochemistry-Paraffin.

Specifications

• Antigen: Golgi Glycoprotein 1/GLG1
• Classification: Monoclonal
• Concentration: 0.2mg/mL
• Dilution: Western Blot 0.5 - 1.0 ug/ml, Flow Cytometry 0.5 - 1 ug/million cells in 0.1ml, Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml, Immunofluorescence 0.5 - 1.0 ug/ml, Immunocytochemistry 0.5 - 1.0 ug/ml
• Gene Accession No.: Q92896, Q92896
• Gene Symbols: GLG1
• Immunogen: Golgi fraction from human liver cells
• Purification Method: Protein A or G purified
• Regulatory Status: RUO
• Gene ID (Entrez): 2734
• Target Species: Human, Mouse (Negative), Rat (Negative)
• Form: Purified

• Applications: Western Blot, Flow Cytometry, Immunohistochemistry (Paraffin), Immunofluorescence, Immunocytochemistry
• Clone: GLG1/970
• Conjugate: Unconjugated
• Formulation: 10mM PBS and 0.05% BSA with 0.05% Sodium Azide
• Gene Alias: CFR1, CFR-1, cysteine-rich fibroblast growth factor receptor, DKFZp686L08213, E-selectin ligand 1, ESL1, ESL-1, FLJ23319, FLJ23967, FLJ32797, Golgi apparatus protein 1, golgi glycoprotein 1, golgi sialoglycoprotein MG-160, MG160, MG-160
• Host Species: Mouse
• Molecular Weight of Antigen: 134 kDa
• Quantity: 0.02 mg
• Primary or Secondary: Primary
• Test Specificity: This MAb recognizes a protein of 134kDa, which binds fibroblast growth factor and E-selectin (cell-adhesion lectin on endothelial cells mediating the binding of neutrophils). Fucosylation is essential for binding to E-selectin. It contains sialic acid residues and 16 Cys-rich GLG1 repeats. This MAb can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region and trans face. Each region consists of various enzymes that selectively modify the macromolecules passing though them, depending on where they are destined to reside. Several spherical vesicles that have budded off of the Golgi are present surrounding the main cisternae. The Golgi tends to be more pronounced and numerous in cells that make and secrete many substances such as plasma B cells.
• Content And Storage: Store at 4C.
• Isotype: IgG1 κ

For Research Use Only