GST Monoclonal Antibody (2F10B9), THE™, GenScript™

Mouse Monoclonal Antibody

Catalog Number	Mfr. No.	Quantity
50-272-995	Genscript Corporation A00865100	100 μg
50-272-997	Genscript Corporation A00865200	200 μg
50-272-998	Genscript Corporation A00865500	500 μg

Description

Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/mL. Schistosoma japonicum glutathione S-transferase (GST) is a 26,000 Da enzyme that conjugates reduced glutathione to hydrophobic electrophiles. GST is an ideal fusion partner for production of foreign proteins in commercially available expression systems because it can be expressed in high levels in E. coli. The fusion proteins can be purified to homogenity in a single step as the GST portion of the protein binds tightly to immobilized glutathione. Elution can be accomplished using free glutathione. The GST portion of the fusion protein can be separated from the protein of interest by using site-specific proteases. Antibodies directed against GST can be helpful in detecting the fusion protein during purification and to detect cleavage of GST from the protein of interest.

GST Monoclonal antibody specifically detects GST in Tag samples. It is validated for ELISA, Immunoprecipitation, Western Blot

Specifications

• Antigen: GST
• Applications: ELISA, Immunoprecipitation, Western Blot
• Conjugate: Unconjugated
• Host Species: Mouse
• Formulation: PBS with 0.02% sodium azide; pH 7.4
• Immunogen: Recombinant GST protein (26 kD)
• Primary or Secondary: Primary
• Content And Storage: -20°C or -80°C if preferred

• Clone: 2F10B9
• Classification: Monoclonal
• Form: Lyophilized
• Regulatory Status: RUO
• Gene Alias: activator protein 1; AP1; AP-1; cancer/testis antigen 48; c-Jun; CT48; deafness, X-linked 7; DFN7; enhancer-binding protein AP1; epididymis secretory protein Li 22; FAEES3; fatty acid ethyl ester synthase III; glutathione S-alkyltransferase; glutathione S-aralkyltransferase; glutathione S-aryltransferase; glutathione S-transferase M1; glutathione S-transferase mu 1; Glutathione S-transferase P; glutathione S-transferase pi 1; Glutathione-S-Transferase; gonad-specific transporter; GST; GST class-mu 1; GST class-pi; GST HB subunit 4; GST protein; GST Tag; GST1; GST3; GSTM1; GSTM1-1; GSTM1a-1a; GSTM1b-1b; GSTP; GSTP1; GSTP1-1; GTH4; GTM1; H-B; HB subunit 4; HEL-S-22; JUN; Jun activation domain binding protein; jun oncogene; jun proto-oncogene; Jun proto-oncogene, AP-1 transcription factor subunit; MU; MU-1; OATP6A1; OATP-I; OATPY; Organic anion-transporting polypeptide 6A1; Organic anion-transporting polypeptide I; p39; PI; Proto-oncogene c-Jun; S-(hydroxyalkyl)glutathione lyase; SLC21A19
• Isotype: IgG1 κ
• Purification Method: Protein A
• Product Type: Antibody