HIF-1 alpha Antibody - BSA Free, Novus Biologicals™

Rabbit Polyclonal Antibody has been used in 175 publications

Supplier:  Novus Biologicals NB100134

Catalog No. NB100134

Description

HIF-1 alpha Polyclonal specifically detects HIF-1 alpha in Human, Mouse, Rat, Bovine, Canine, Guinea Pig, Primate, Xenopus, Zebrafish samples. It is validated for Western Blot, Simple Western, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunoblotting, Gel Super Shift Assays, Chromatin Immunoprecipitation (ChIP), Knockout Validated, Chromatin Immunoprecipitation Sequencing, Dual RNAscope ISH-IHC.

Specifications

• Antigen: HIF-1 alpha
• Classification: Polyclonal
• Conjugate: Unconjugated
• Formulation: PBS with 0.02% Sodium Azide
• Gene Alias: AINT, anti-HIF-1 alpha, anti-HIF1A, ARNT interacting protein, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, BHLHE78, Class E basic helix-loop-helix protein 78, HIF1, HIF-1 alpha, HIF1A, HIF-1A, HIF-1alpha, HIF-1-alpha, HIF1-alpha, hyp
• Host Species: Rabbit
• Molecular Weight of Antigen: 93 kDa
• Quantity: 0.1 mL
• Research Discipline: Adaptive Immunity, Angiogenesis, Autophagy, Cancer, Cardiovascular Biology, Cellular Markers, Chromatin Research, Cytokine Research, Hypoxia, Hypoxia Inducible Factors (HIF), Hypoxia Signaling, Immunology, Inflammation, Innate Immunity, Lipid and Metabolism, mTOR Pathway, Signal Transduction, Transcription Factors and Regulators
• Gene ID (Entrez): 3091
• Target Species: Human, Mouse, Rat, Bovine, Canine, Guinea Pig, Primate, Xenopus species, Zebrafish
• Isotype: IgG

• Applications: ChIP Assay, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen), Immunoblot, Gel Shift, ChIP Assay, Immunoassay
• Concentration: 1.0 mg/mL
• Dilution: Western Blot 1:500 - 1:1000, Simple Western 1:100, ELISA, Immunohistochemistry 1:100 - 1:500, Immunocytochemistry/Immunofluorescence, Immunoprecipitation 1:1000, Immunohistochemistry-Paraffin 1:100 - 1:500, Immunohistochemistry-Frozen 1:100 - 1:500, Immunoblotting, Gel Super Shift Assays 1:1 - 1:100, Chromatin Immunoprecipitation (ChIP), Knockout Validated, Chromatin Immunoprecipitation Sequencing reported in scientific literature (PMID 34277635), Dual RNAscope ISH-IHC
• Gene Accession No.: Q61221, O35800
• Gene Symbols: HIF1A
• Immunogen: This HIF-1 alpha Antibody was developed against a fusion protein made to an internal sequence of human HIF-1 alpha (containing amino acids 432 - 528) [Uniprot# Q16665].
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Human, monkey, mouse, rat and Xenopus. Canine reactivity reported in scientific literature (PMID: 24140705) Mouse results are mixed.
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

For Research Use Only