HIF-1 alpha Antibody - BSA Free, Novus Biologicals™

Rabbit Polyclonal Antibody has been used in 13 publications

Supplier:  Novus Biologicals NB100654SS

Catalog No. NB100654SS

Description

HIF-1 alpha Polyclonal specifically detects HIF-1 alpha in Human, Mouse, Rat, Porcine, Bovine samples. It is validated for Western Blot, Simple Western, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Gel Super Shift Assays, Knockdown Validated.

Specifications

• Antigen: HIF-1 alpha
• Classification: Polyclonal
• Conjugate: Unconjugated
• Formulation: PBS with 0.02% Sodium Azide
• Gene Alias: AINT, anti-HIF-1 alpha, anti-HIF1A, ARNT interacting protein, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, BHLHE78, Class E basic helix-loop-helix protein 78, HIF1, HIF-1 alpha, HIF1A, HIF-1A, HIF-1alpha, HIF-1-alpha, HIF1-alpha, hyp
• Host Species: Rabbit
• Molecular Weight of Antigen: 93 kDa
• Quantity: 0.025 mL
• Research Discipline: Adaptive Immunity, Angiogenesis, Autophagy, Cancer, Cardiovascular Biology, Cellular Markers, Chromatin Research, Cytokine Research, Hypoxia, Hypoxia Inducible Factors (HIF), Hypoxia Signaling, Immunology, Inflammation, Innate Immunity, Lipid and Metabolism, mTOR Pathway, Signal Transduction, Transcription Factors and Regulators
• Gene ID (Entrez): 3091
• Target Species: Human, Mouse, Rat, Pig, Bovine
• Isotype: IgG

• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen), Gel Shift, KnockDown
• Concentration: 1.0 mg/mL
• Dilution: Western Blot 1:1000, Simple Western 1:100, Immunohistochemistry 1:100 - 1:300, Immunocytochemistry/Immunofluorescence 1:10 - 1:500. Use reported in scientific literature, Immunohistochemistry-Paraffin 1:100 - 1:300. Use reported in scientific literature, Immunohistochemistry-Frozen 1:100 - 1:300. Use reported in scientific literature, Gel Super Shift Assays, KnockDown Validated reported in scientific literature (PMID 32054096)
• Gene Accession No.: Q16665
• Gene Symbols: HIF1A
• Immunogen: This HIF-1 alpha Antibody was developed against a fusion protein containing amino acids 432 - 528 of human HIF-1 alpha [Uniprot# Q16665].
• Purification Method: Affinity Purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Human and porcine. Does not react with rat. Immunogen sequence has 93% homology to bovine and 90% homology to mouse.Mouse reactivity reported in scientific literature (PMID: 23959856) Rat reactivity reported in scientific literature (PMID: 24122166).
• Content And Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

For Research Use Only