HIF-1 alpha Antibody (ESEE122), Novus Biologicals™

Mouse Monoclonal Antibody has been used in 81 publication

Supplier:  Novus Biologicals NB100131

Catalog No. NB100131

Description

HIF-1 alpha Monoclonal specifically detects HIF-1 alpha in Human, Mouse, Rat, Bovine, Canine samples. It is validated for Western Blot, Simple Western, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunoblotting.

Specifications

• Antigen: HIF-1 alpha
• Classification: Monoclonal
• Concentration: 1.0 mg/mL
• Dilution: Western Blot 1:500-1:1000, Simple Western 1:2000, Flow Cytometry reported in scientific literature (Gestier S. et al), Immunohistochemistry 1:100-1:5000, Immunocytochemistry/Immunofluorescence 1:100, Immunoprecipitation 1:10-1:500. Use reported in scientific literature (PMID 26757928 Fig1G), Immunohistochemistry-Paraffin 1:100-1:5000, Immunohistochemistry-Frozen 1:100-1:5000, Immunoblotting
• Gene Alias: AINT, anti-HIF-1 alpha, anti-HIF1A, ARNT interacting protein, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, BHLHE78, Class E basic helix-loop-helix protein 78, HIF1, HIF-1 alpha, HIF1A, HIF-1A, HIF-1alpha, HIF-1-alpha, HIF1-alpha, hyp
• Host Species: Mouse
• Molecular Weight of Antigen: 93 kDa
• Quantity: 0.1 mL
• Research Discipline: Adaptive Immunity, Angiogenesis, Autophagy, Cancer, Cardiovascular Biology, Cellular Markers, Chromatin Research, Cytokine Research, Hypoxia, Hypoxia Inducible Factors (HIF), Hypoxia Signaling, Immunology, Inflammation, Innate Immunity, Lipid and Metabolism, mTOR Pathway, Signal Transduction, Transcription Factors and Regulators
• Gene ID (Entrez): 3091
• Target Species: Human, Mouse, Rat, Bovine, Canine
• Form: Purified

• Applications: Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen), Immunoblot
• Clone: ESEE122
• Conjugate: Unconjugated
• Gene Accession No.: Q16665
• Gene Symbols: HIF1A
• Immunogen: This HIF-1 alpha Antibody (ESEE122) was developed against Human HIF-1 alpha, corresponding to amino acids 329 - 530 [Uniprot# Q16665].
• Purification Method: Protein G purified
• Regulatory Status: RUO
• Primary or Secondary: Primary
• Test Specificity: Stains HIF-1 alpha in free-floating canine brain sections, according to customer reports.
• Content And Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Isotype: IgG1

For Research Use Only