HIF-1 alpha Antibody (ESEE122), Novus Biologicals™

Mouse Monoclonal Antibody has been used in 81 publication

Catalog Number	Mfr. No.	Quantity
NB100131SS	Novus Biologicals NB100131SS	0.025 mL
NB100131	Novus Biologicals NB100131	0.1 mL

Description

HIF-1 alpha Monoclonal specifically detects HIF-1 alpha in Human, Mouse, Rat, Bovine, Canine samples. It is validated for Western Blot, Simple Western, Flow Cytometry, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunoblotting.

Specifications

• Antigen: HIF-1 alpha
• Concentration: 1.0 mg/mL
• Applications: Western Blot, Flow Cytometry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen), Immunoblot
• Conjugate: Unconjugated
• Host Species: Mouse
• Research Discipline: Adaptive Immunity, Angiogenesis, Autophagy, Cancer, Cardiovascular Biology, Cellular Markers, Chromatin Research, Cytokine Research, Hypoxia, Hypoxia Inducible Factors (HIF), Hypoxia Signaling, Immunology, Inflammation, Innate Immunity, Lipid and Metabolism, mTOR Pathway, Signal Transduction, Transcription Factors and Regulators
• Gene Accession No.: Q16665
• Gene ID (Entrez): 3091
• Immunogen: This HIF-1 alpha Antibody (ESEE122) was developed against Human HIF-1 alpha, corresponding to amino acids 329 - 530 [Uniprot# Q16665].
• Primary or Secondary: Primary
• Content And Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Molecular Weight of Antigen: 93 kDa

• Clone: ESEE122
• Dilution: Western Blot 1:500-1:1000, Simple Western 1:2000, Flow Cytometry reported in scientific literature (Gestier S. et al), Immunohistochemistry 1:100-1:5000, Immunocytochemistry/Immunofluorescence 1:100, Immunoprecipitation 1:10-1:500. Use reported in scientific literature (PMID 26757928 Fig1G), Immunohistochemistry-Paraffin 1:100-1:5000, Immunohistochemistry-Frozen 1:100-1:5000, Immunoblotting
• Classification: Monoclonal
• Form: Purified
• Regulatory Status: RUO
• Target Species: Human, Mouse, Rat, Bovine, Canine
• Gene Alias: AINT, anti-HIF-1 alpha, anti-HIF1A, ARNT interacting protein, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, BHLHE78, Class E basic helix-loop-helix protein 78, HIF1, HIF-1 alpha, HIF1A, HIF-1A, HIF-1alpha, HIF-1-alpha, HIF1-alpha, hyp
• Gene Symbols: HIF1A
• Isotype: IgG1
• Purification Method: Protein G purified
• Test Specificity: Stains HIF-1 alpha in free-floating canine brain sections, according to customer reports.

For Research Use Only