HIF-1 alpha Antibody (ESEE122) - Azide and BSA Free, Novus Biologicals™

Catalog No.	Quantity
NB013835	0.1 mL

Catalog No. NB013835 Supplier Novus Biologicals Supplier No. NBP280762

Description

Mouse Monoclonal Antibody

HIF-1 alpha Monoclonal specifically detects HIF-1 alpha in Human, Mouse, Rat, Bovine, Canine samples. It is validated for Western Blot, Simple Western, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Immunoblotting, Immunocytochemistry.

Specifications

• Antigen: HIF-1 alpha
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (Paraffin), Immunohistochemistry (Frozen), Immunoblot
• Classification: Monoclonal
• Clone: ESEE122
• Concentration: 1 mg/mL
• Conjugate: Unconjugated
• Dilution: Western Blot 1:500 - 1:1000, Simple Western : 1:2000, Immunohistochemistry, Immunocytochemistry/Immunofluorescence : 1:100, Immunoprecipitation 1:10 - 1:500. Use reported in scientific literature (PMID 26757928 Fig1G), Immunohistochemistry-Paraffin : 1:100 - 1:5000, Immunohistochemistry-Frozen : 1:100 - 1:5000, Immunoblotting, Immunocytochemistry
• Formulation: Tris-Glycine, 0.15M NaCl
• Gene Alias: AINT, anti-HIF-1 alpha, anti-HIF1A, ARNT interacting protein, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, BHLHE78, Class E basic helix-loop-helix protein 78, HIF1, HIF-1 alpha, HIF1A, HIF-1A, HIF-1alpha, HIF-1-alpha, HIF1-alpha, hypoxia inducible factor 1 alpha subunit, hypoxia inducible factor 1 subunit alpha, hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor), hypoxia-inducible factor 1-alpha, Member of PAS protein 1, member of PAS superfamily 1, MOP1, PAS domain-containing protein 8, PASD8
• Gene Symbols: HIF1A
• Host Species: Mouse
• Immunogen: This HIF-1 alpha Antibody (ESEE122) was developed against Human HIF-1 alpha, corresponding to amino acids 329 - 530 [Uniprot# Q16665].
• Purification Method: Protein G purified
• Quantity: 0.1 mL
• Regulatory Status: RUO
• Research Discipline: Adaptive Immunity, Angiogenesis, Autophagy, Cancer, Cardiovascular Biology, Cell Biology, Cell Cycle and Replication, Cellular Markers, Chromatin Research, Cytokine Research, HIF Target Genes, Hypoxia, Hypoxia Inducible Factors (HIF), Hypoxia Signaling, Immunology, Inflammation, Innate Immunity, Lipid and Metabolism, mTOR Pathway, Signal Transduction, Transcription Factors and Regulators
• Primary or Secondary: Primary
• Gene ID (Entrez): 3091
• Test Specificity: Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
• Target Species: Human, Mouse, Rat, Bovine, Canine
• Content And Storage: Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
• Form: Purified
• Isotype: IgG1