HIF-1 alpha Antibody (H1alpha67), Novus Biologicals™

Catalog No.	Quantity
NB100105	0.1 mL
NB100105SS	0.025 mL

Catalog No. NB100105 Supplier Novus Biologicals Supplier No. NB100105

Description

Mouse Monoclonal Antibody has been used in 891 publication

HIF-1 alpha Monoclonal specifically detects HIF-1 alpha in Bovine, Canine, Feline, Ferret, Human, Monkey, Mouse, Ovine, Pig, Primate, Rabbit, Rat, Sheep, Xenopus species, Xenopus species (African Clawed Frog) samples. It is validated for Chemotaxis, ChIP Assay, ELISA, EMSA, Flow Cytometry, Gel Super Shift Assays, Gel Supershift, Gene Knock-Out, IHC/IF, Immunoassay, Immunoblot, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Free Floating), Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, In vitro Assay, KnockDown, Ligand Activation, Proximity Ligation Assay, SDS-Page, Simple Western, Tissue Culture Substratum, Western Blot.

Specifications

• Antigen: HIF-1 alpha
• Applications: ChIP Assay, ELISA, Flow Cytometry, Gel Supershift, Gene Knock-Out, Immunoassay, Immunoblot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry (Free Floating), Immunohistochemistry (Frozen), Immunohistochemistry (Paraffin), Immunoprecipitation, In vitro Assay, KnockDown, Ligand Activation, Proximity Ligation Assay, Simple Western, Tissue Culture Substratum, Western Blot
• Classification: Monoclonal
• Clone: H1alpha67
• Concentration: 1.0 mg/mL
• Conjugate: Unconjugated
• Dilution: Western Blot 1:500, Simple Western, Chromatin Immunoprecipitation 1 - 5 ug/IP. Use reported in scientific literature, Flow Cytometry 1:10 - 1:1000, ELISA 1:100 - 1:2000. Use reported in scientific literature (PMID 20042684), Immunohistochemistry 1:20-1:50, Immunocytochemistry/Immunofluorescence 1:50, Immunoprecipitation 1:10-1:500, Immunohistochemistry-Paraffin 1:20-1:50, Immunohistochemistry-Frozen 1:20-1:50, Immunoassay reported in scientific literature (PMID 26147748), Immunoblotting reported in multiple pieces of scientific literature, In vitro assay reported in multiple pieces of scientific literature, Gel Super Shift Assays 1:1 - 1:100. Use reported in scientific literature (PMID 22411794), Proximity Ligation Assay reported in scientific literature (PMID 27595394), Tissue Culture Substratum, Ligand Activation reported in scientific literature (PMID 26147748), Immunohistochemistry Free-Floating reported in scientific literature (PMID 33242463), Chromatin Immunoprecipitation (ChIP) 1-5 ug/IP, Knockout Validated reported in scientific literature (PMID 26861754), KnockDown Validated reported in scientific literature (PMID 32772041)
• Gene Accession No.: Q16665
• Gene Alias: AINT, anti-HIF-1 alpha, anti-HIF1A, ARNT interacting protein, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, BHLHE78, Class E basic helix-loop-helix protein 78, H1alpha67, HIF1, HIF-1 alpha, HIF1A, HIF-1A, HIF-1alpha, HIF-1-alpha, HIF1
• Gene Symbols: HIF1A
• Host Species: Mouse
• Immunogen: This HIF-1 alpha Antibody (H1alpha67) was developed against a fusion protein containing amino acids 432 - 528 of human HIF-1 alpha [Uniprot# Q16665].
• Molecular Weight of Antigen: 93 kDa
• Purification Method: Protein G purified
• Quantity: 0.1 mL
• Regulatory Status: RUO
• Research Discipline: Adaptive Immunity, Angiogenesis, Autophagy, Cancer, Cardiovascular Biology, Cellular Markers, Chromatin Research, Cytokine Research, Hypoxia, Hypoxia Inducible Factors (HIF), Hypoxia Signaling, Immunology, Inflammation, Innate Immunity, Lipid and Metabolism, mTOR Pathway, Signal Transduction, Transcription Factors and Regulators
• Primary or Secondary: Primary
• Gene ID (Entrez): 3091
• Test Specificity: Reactivity with Candida albicans reported by a customer review. Reactivity with Xenopus reported in the scientific literature (PMID: 18303027).
• Target Species: Bovine, Mouse, Canine, Feline, Ferret, Canine, Human, Ferret, Monkey, Primate, Rabbit, Mouse, Ovine, Pig, Primate, Rabbit, Rat, Sheep, Xenopus species, Xenopus species
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Form: Purified
• Isotype: IgG2b

For Research Use Only