HIF-1 alpha Antibody (H1alpha67), Novus Biologicals™

Catalog No.	Quantity
NB100123	0.1 mL
NB100123SS	0.025 mL

Catalog No. NB100123 Supplier Novus Biologicals Supplier No. NB100123

Description

Mouse Monoclonal Antibody

HIF-1 alpha Monoclonal specifically detects HIF-1 alpha in Human, Mouse, Rat, Porcine, Avian, Bovine, Canine, Ferret, Primate, Rabbit, Sheep samples. It is validated for Western Blot, Chromatin Immunoprecipitation, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry/Immunofluorescence, Immunoprecipitation, Immunohistochemistry-Paraffin, Immunoblotting, Gel Super Shift Assays, Knockout Validated, Knockdown Validated.

Specifications

• Antigen: HIF-1 alpha
• Applications: Western Blot, ChIP Assay, Flow Cytometry, ELISA, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Classification: Monoclonal
• Clone: H1alpha67
• Concentration: 1.0 mg/mL
• Conjugate: Unconjugated
• Dilution: Western Blot 1:500 - 1:1000, Chromatin Immunoprecipitation 1:10 - 1:500. Use reported in scientific literature, Flow Cytometry 1:10 - 1:1000, ELISA 1:100 - 1:2000. Use reported in scientific literature, Immunohistochemistry 1:100 - 1:300, Immunocytochemistry/Immunofluorescence, Immunoprecipitation 1:10, Immunohistochemistry-Paraffin 1:100 - 1:300, Immunoblotting reported in multiple pieces of scientific literature, Gel Super Shift Assays 1:1 - 1:100. Use reported in scientific literature, Chromatin Immunoprecipitation (ChIP) 1:10-1:500, Knockout Validated reported in scientific literature (PMID 27991597), KnockDown Validated
• Gene Accession No.: Q16665
• Gene Alias: AINT, anti-HIF-1 alpha, anti-HIF1A, ARNT interacting protein, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, BHLHE78, Class E basic helix-loop-helix protein 78, H1alpha67, HIF1, HIF-1 alpha, HIF1A, HIF-1A, HIF-1alpha, HIF-1-alpha, HIF1
• Gene Symbols: HIF1A
• Host Species: Mouse
• Immunogen: This HIF-1 alpha Antibody (H1alpha67) was developed against a fusion protein containing amino acids 432 - 528 of human HIF-1 alpha [Uniprot# Q16665].
• Molecular Weight of Antigen: 93 kDa
• Purification Method: Protein A purified
• Quantity: 0.1 mL
• Regulatory Status: RUO
• Research Discipline: Adaptive Immunity, Angiogenesis, Autophagy, Cancer, Cardiovascular Biology, Cellular Markers, Chromatin Research, Cytokine Research, Hypoxia, Hypoxia Inducible Factors (HIF), Hypoxia Signaling, Immunology, Inflammation, Innate Immunity, Lipid and Metabolism, mTOR Pathway, Signal Transduction, Transcription Factors and Regulators
• Primary or Secondary: Primary
• Gene ID (Entrez): 3091
• Test Specificity: Recognizes human, mouse, rat, bovine, monkey, porcine, sheep, canine, bird and ferret HIF-1 alpha.
• Target Species: Human, Mouse, Rat, Pig, Avian, Bovine, Canine, Ferret, Primate, Sheep
• Content And Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Form: Purified
• Isotype: IgG2b

For Research Use Only